Summary
In cats the intravenous injection of a substance enriched from stored serum (DAS=depressor active substance) led to an extreme increase in pulmonary vascular resistance (PVR) and at the same time to a marked decrease in platelet count. The maximum increase in PVR was significantly related to the platelet count. The histologic examination of lung tissue—taken at the time of maximum increase in PVR—revealed an extensive microembolism of the terminal pulmonary vessels by platelet aggregates.
DAS proved more active than adenosine diphosphate (ADP) from which it can be distinguished by its solubility in organic solvents and by a typical tachyphylactic response.
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Schumacher, K.A., Classen, H.G. Platelet aggregation and increased pulmonary vascular resistance in cats induced by DAS and ADP. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 373–381 (1972). https://doi.org/10.1007/BF00501126
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DOI: https://doi.org/10.1007/BF00501126