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Rotational behaviour and cGMP responses following manipulation of nigral mechanisms with chlordiazepoxide

Evidence for Enhancement of GABA Transmission by Benzodiazepines

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Summary

Unilateral stereotaxic injections of 1 μg of the soluble benzodiazepine chlordiazepoxide hydrochloride into the predominantly GABA-containing zona reticulata of the substantia nigra of amphetamine-pretreated rats induced rotational behaviour similar to that seen following unilateral elevation of nigral GABA levels and amphetamine treatment; this effect was not seen following injections into the vicinity of the predominantly dopamine-containing zona compacta. Chlordiazepoxide-induced rotations were abolished by the GABA-antagonist picrotoxin. Both chlordiazepoxide and GABA depressed production of cyclic 3′,5′-guanosine monophosphate in samples of nigral tissue in vitro as estimated by radioimmunoassay. It is concluded that chlordiazepoxide may enhance GABA transmission within the substantia nigra, by some as yet unidentified mechanism, to create asymmetric activity in GABA-modulated neurones and hence induce rotation.

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Authors and Affiliations

  1. Division of Psychiatry, MRC Clinical Research Centre, HA1 3UJ, GB-Harrow, Middlesex, UK

    John L. Waddington & Adrian Longden

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  1. John L. Waddington
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  2. Adrian Longden
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Waddington, J.L., Longden, A. Rotational behaviour and cGMP responses following manipulation of nigral mechanisms with chlordiazepoxide. Naunyn-Schmiedeberg's Arch. Pharmacol. 300, 233–237 (1977). https://doi.org/10.1007/BF00500965

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  • DOI: https://doi.org/10.1007/BF00500965

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