Studies on the variables and kinetics of SCP production from nopal fruit juice

  • S. N. Mukhopadhyay
  • A. Ornelas Vale
  • E. Camargo Rubio
  • G. Cassarubias Arcos
Industrial Microbiology


Submerged batch cultivation under controlled environmental conditions of pH 3.8, temperature 30°C, and KLa≥200 h−1 (above 180 mMO2l−1 h−1 oxygen supply rate) produced a maximum (12.0 g·l−1) SCP (Candida utilis) yield on the deseeded nopal fruit juice medium containing C/N ratio of 7.0 (initial sugar concentration 25 g·l−1) with a yield coefficient of 0.52 g cells/g sugar. In continuous cultivation, 19.9 g·l−1 cell mass could be obtained at a dilution rate (D) of 0.36 h−1 under identical environmental conditions, showing a productivity of 7.2 g·l−1·h−1. This corresponded to a gain of 9.0 in productivity in continuous culture over batch culture. Starting with steady state values of state variables, cell mass (CX−19.9 g·l−1), limiting nutrient concentration (Cln−2.5 g·l−1) and sugar concentration (CS−1.5 g·l−1) at control variable conditions of pH 3.8, 30°C, and KLa 200 h−1 keeping D=0.36 h−1 as reference, transient response studies by step changes of these control variables also showed that this pH, temperature and KLa conditions are most suitable for SCP cultivation on nopal fruit juice. Kinetic equations obtained from experimental data were analysed and kinetic parameters determined graphically. Results of SCP production from nopal fruit juice are described.


Candida Cell Mass Batch Cultivation Dilution Rate Continuous Cultivation 
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concentration of ammonium sulfate (g·l−1)


concentration of total sugar (g·l−1)


cell concentration (g·l−1)


dilution rate (h−1)


Monod's constant (g·l−1)


maintenance coefficient (g ammonium sulfate cell−1 h−1)


maintenance coefficient (g sugar g cell−1 h−1)


time, h


yield coefficient (g cells/g ammonium sulfate)


maximum of Y


yield coefficient based on sugar consumed (g cells · g sugar−1)


maximum value of YS


maximum specific growth rate constant (h−1)


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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • S. N. Mukhopadhyay
    • 1
  • A. Ornelas Vale
    • 1
  • E. Camargo Rubio
    • 1
  • G. Cassarubias Arcos
    • 1
  1. 1.Biotechnology DepartmentLaboratorios Nacionales de Fomento IndustrialMexico 10

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