A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass

  • G. Braunegg
  • B. Sonnleitner
  • R. M. Lafferty
Biotechnology and Bioengineering


The gas chromatographic method for the determination of poly-β-hydroxybutyric acid (PHB) consists of a mild acid or alkaline methanolysis of poly-β-hydroxybutyric acid directly without previous extraction of PHB from the cells; this is followed by gas chromatography of the 3-hydroxybutyric acid methylester. The method is characterized by high accuracy and excellent reproducibility, permitting determinations as low as 10−5 g/l. Only 4 h is required from sampling from the fermenter till completion of the PHB determination.


Biomass Chromatography Microbial Biomass Chromatographic Method Excellent Reproducibility 
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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • G. Braunegg
    • 1
  • B. Sonnleitner
    • 1
  • R. M. Lafferty
    • 1
  1. 1.Institut für Biochemische Technologie und Lebensmittelchemie der Technischen Universität GrazGrazAustria

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