Effect of different environmental parameters on the biofilm build-up of Pseudomonas putida ATCC 11172 in chemostat

  • Göran Molin
  • Inge Nilsson


The influence of carbon source, cell concentration, oxygen tension, pH and temperature on the biofilm build-up of Pseudomonas putida ATCC 11172 was studied. The experiments were performed in a carbon and energy limited chemostat (asparagine). When the asparagine was replaced by glucose the biofilm build-up was decreasing.

Cell concentration, oxygen tension or temperature did not to any significant degree affect the biofilm build-up. Temperature, however influenced the size of the suspended cells. The cell size successively increased with decreasing temperature. A similar change in cell size could be accomplished by increasing the dilution rate from 0.2 h−1 (small cells) to 0.7 h−1 (large cells).

The biofilm build-up increased with increasing pH in the interval of 5.5–6.7. The viable count of the biofilm after 24 h exposure of test surface increased from 8×106 cfu/cm2 to 1.6×108 cfu/cm2 while the biomass increased by a factor of about 70.


Oxygen Glucose Biomass Carbon Source Suspended Cell 
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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Göran Molin
    • 1
  • Inge Nilsson
    • 2
  1. 1.Swedish Meat Research InstituteKävlingeSweden
  2. 2.Department of Applied Microbiology, Chemical CenterUniversity of LundLundSweden

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