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Effect of different environmental parameters on the biofilm build-up of Pseudomonas putida ATCC 11172 in chemostat

  • Göran Molin
  • Inge Nilsson
Environmental

Summary

The influence of carbon source, cell concentration, oxygen tension, pH and temperature on the biofilm build-up of Pseudomonas putida ATCC 11172 was studied. The experiments were performed in a carbon and energy limited chemostat (asparagine). When the asparagine was replaced by glucose the biofilm build-up was decreasing.

Cell concentration, oxygen tension or temperature did not to any significant degree affect the biofilm build-up. Temperature, however influenced the size of the suspended cells. The cell size successively increased with decreasing temperature. A similar change in cell size could be accomplished by increasing the dilution rate from 0.2 h−1 (small cells) to 0.7 h−1 (large cells).

The biofilm build-up increased with increasing pH in the interval of 5.5–6.7. The viable count of the biofilm after 24 h exposure of test surface increased from 8×106 cfu/cm2 to 1.6×108 cfu/cm2 while the biomass increased by a factor of about 70.

Keywords

Oxygen Glucose Biomass Carbon Source Suspended Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Göran Molin
    • 1
  • Inge Nilsson
    • 2
  1. 1.Swedish Meat Research InstituteKävlingeSweden
  2. 2.Department of Applied Microbiology, Chemical CenterUniversity of LundLundSweden

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