Production of vitamin B12 by immobilized cells of a propionic acid bacterium

Summary

The ability of immobilized cells of propionic acid bacteria to form vitamin B12 has been investigated. Propionibacterium arl AKU 1251 having a considerable activity to produce the vitamin was selected as a test organism among six strains of propionic acid bacteria tested. The whole cells were entrapped with urethane prepolymers, photo-crosslinkable resin prepolymers or several other materials such as κ-carrageenan, agar or sodium alginate, and their vitamin B12 productivity was compared. Based on the criteria of the convenience of preparation and the stability of the cell-entrapping gels, a hydrophilic urethane prepolymer, PU-9, was employed as gel material. Satisfactory vitamin B12 production was obtained when 5–10 g of wet cells precultured to the late exponential growth phase were entrapped with 1 g of the prepolymer. Addition of a suitable amount of cobaltous ion and of 5,6-dimethyl benzimidazole to the culture medium was effective for the production of the vitamin by the immobilized cells. The repeated use of the immobilized cells was successfully achieved when a suitable amount of cells were entrapped and allowed the proliferation of cells inside gel matrices.

This is a preview of subscription content, access via your institution.

References

  1. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Google Scholar 

  2. Friedrich W, Bernhauer K (1954) Über das Verhalten der Vitamine der B12-Gruppe gegenüber Phenolen. Z Naturwiss 96:754–761

    Google Scholar 

  3. Fukui S, Omata T, Yamane T, Tanaka A (1980a) Application of immobilized biocatalysts to bioconversion in hydrophobic conditions. Enzyme Engineering 5:347–353

    Google Scholar 

  4. Fukui S, Sonomoto K, Itoh N, Tanaka A (1980b) Several novel methods for immobilization of enzymes, microbial cells and organelles. Biochimie 62:381–386

    Google Scholar 

  5. Fukui S, Tanaka A (1981) Bioconversion of lipophilic compounds by immobilized microbial cells in organic solvents. Acta Biotechnol 1:339–350

    Google Scholar 

  6. Fukui S, Tanaka A (1982) Immobilized microbial cells. Ann Rev Microbiol 36:145–172

    Google Scholar 

  7. Fukui S, Tanaka A, Iida T, Hasegawa E (1976) Application of photo-crosslinkable resin to immbolization of an enzyme. FEBS Lett 66:179–182

    Google Scholar 

  8. Fukushima S, Nagai T, Fujita K, Tanaka A, Fukui S (1978) Hydrophilic urethane prepolymers: Convenient materials for enzyme entrapment. Biotechnol Bioeng 20:1465–1469

    Google Scholar 

  9. Omata T, Iida T, Tanaka A, Fukui S (1978) Transformation of steroids by gel-entrapped Nocardia rhodocrous cells in organic solvent. Eur J Appl Microbiol Biotechnol 8:143–155

    Google Scholar 

  10. Skeggs HR (1967) Vitamin B12. In: György P, Pearson WN (eds) The vitamins — Chemistry, Physiology, Pathology, Methods, vol 7. Academic Press, New York London, pp 277–301

    Google Scholar 

  11. Sonomoto K, Jin I-N, Tanaka A, Fukui S (1980) Application of urethane prepolymers to immobilization of biocatalysts: Δ1-Dehydrogeneration of hydrocortisone by Arthrobacter simplex cells entrapped with urethane prepolymers. Agric Biol Chem 44:1119–1126

    Google Scholar 

  12. Sonomoto K, Tanaka A, Omata T, Yamane T, Fukui S (1979) Application of photo-crosslinkable resin prepolymers to entrap microbial cells. Effect of cell-entrapping gel hydrophobicity on the hydrocortisone Δ1-dehydrogeneration. Eur J Appl Microbiol Biotechnol 6:325–334

    Google Scholar 

  13. Tanaka A, Hagi N, Yasuhara S, Fukui S (1978) Immobilization of catalase with photo-crosslinkable resin prepolymers. J Ferment Technol 56:511–515

    Google Scholar 

  14. Tanaka A, Jin I-N, Kawamoto S, Fukui S (1979) Entrapment of microbial cells and organelles with hydrophilic urethane prepolymers. Eur J Appl Microbiol Biotechnol 7:351–354

    Google Scholar 

  15. Tosa T, Sato T, Mori T, Yamamoto K, Takata I, Nishida Y, Chibata I (1979) Immobilization of enzymes and microbial cells using carrageenan matrix. Biotechnol Bioeng 21:1697–1709

    Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Saburo Fukui.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Yongsmith, B., Sonomoto, K., Tanaka, A. et al. Production of vitamin B12 by immobilized cells of a propionic acid bacterium. European J. Appl. Microbiol. Biotechnol. 16, 70–74 (1982). https://doi.org/10.1007/BF00500729

Download citation

Keywords

  • Alginate
  • Urethane
  • Benzimidazole
  • Sodium Alginate
  • Immobilize Cell