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Histochemistry

, Volume 60, Issue 3, pp 317–320 | Cite as

Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

  • T. F. C. Batten
  • C. R. Hopkins
Article

Summary

ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cell was identified as containing numerous round or ovoid 170–300 nm, secretory granules.

Keywords

Public Health Gold Gold Particle Secretory Granule Ultrathin Section 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • T. F. C. Batten
    • 1
  • C. R. Hopkins
    • 1
  1. 1.Department of Histology and Cell Biology (Medical)University of LiverpoolLiverpoolEngland

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