, Volume 60, Issue 3, pp 317–320 | Cite as

Use of protein A-coated colloidal gold particles for immunoelectronmicroscopic localization of ACTH on ultrathin sections

  • T. F. C. Batten
  • C. R. Hopkins


ACTH was localized in dissociated porcine adenohypophysial cells using a novel indirect EM immunocytochemical technique. Incubation of ultrathin resin sections in anti-ACTH was followed by incubation with protein A-coated colloidal gold particles. Protein A binds specifically to the Fc part of the IgG molecule, and thus the ACTH-containing secretory granules became labelled with electron-dense gold particles. With this method, the dissociated porcine ACTH cell was identified as containing numerous round or ovoid 170–300 nm, secretory granules.


Public Health Gold Gold Particle Secretory Granule Ultrathin Section 
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  1. Bacsy, E., Tougard, C., Tixier-Vidal, A., Marton, J., Stark, E.: Corticotroph cells in primary cultures of rat adenohypophysis: a light-and electron-microscope immunocytochemical study. Histochemistry 50, 161–174 (1976)Google Scholar
  2. Batten, T.F.C., Hopkins, C.R.: Discrimination of LH, FSH, TSH and ACTH in dissociated porcine anterior pituitary cells by light- and electron-microscope immunocytochemistry. Cell Tissue Res. 192, 107–120 (1978)Google Scholar
  3. Biberfeld, P., Ghetie, V., Sjoquist, J.: Demonstration and assaying of IgG antiboidies in tissues and on cells by labeled staphylococcal protein A. J. Immunol. Methods 6, 249–250 (1975)Google Scholar
  4. Dubois-Dalcq, M., McFarland, H., McFarlin, D.: Protein A-peroxidase: a valuable tool for the localization of antigens. J. Histochem. Cytochem. 25, 1201–1206 (1977)Google Scholar
  5. Faulk, W.P., Taylor, G.M.: An immunological method for the electron microscope. Immunochemistry 8, 1081–1083 (1971)Google Scholar
  6. Forsgren, A., Sjoquist, J.: ‘Protein A’ from S. aureus. I. Pseudo-immune reaction with human gamma-globulin. J. Immunol. 97, 822–827 (1966)Google Scholar
  7. Georghegan, W.D., Ackerman, G.A.: Adsorption of horseradish peroxidase, ovomucoid and antiimmunoglobulin to colloidal gold for the indirect detection of concanavalin A, wheat germ agglutinin and goat anti-human immunoblogulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J. Histochem. Cytochem. 25, 1187–1200 (1977)Google Scholar
  8. Horisberger, M., Rosset, J.: Colloidal gold: a useful marker for transmission and scanning electron microscopy. J. Histochem. Cytochem. 25, 295–305 (1977)Google Scholar
  9. Horisberger, M., Rosset, J., Bauer, H.: Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope. Experientia 31, 1147–1149 (1975)Google Scholar
  10. Walker, A.M., Hopkins, C.R.: Dissociation of the porcine anterior pituitary: the kinetics of luteinizing hormone release in response to luteinizing hormone-releasing hormone. Mol. Cell. Endocrinol. 12, 177–187 (1978)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • T. F. C. Batten
    • 1
  • C. R. Hopkins
    • 1
  1. 1.Department of Histology and Cell Biology (Medical)University of LiverpoolLiverpoolEngland

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