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Immunohistochemical localization of glucagon-like peptide 1

Use of poly-and monoclonal antibodies

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Summary

We report the use of poly-and monoclonal antibodies to study the immunohistochemical distribution of glucagon-like peptide-1 immunoreactivity (GLP-1-IR) in various tissues. The polyclonal antibodies against GLP-1 reacted with pancreatic A cells, enteroglucagon (L) cells in the gut, and some neurons in the central nervous system of all species tested. In pancreas and gut the monoclonal antibodies against GLP-1 exhibited a similar, but species specific distribution, relative to the polyclonal antibodies. The colocalization of GLP-1 and glucagon immunoreactivity in pancreatic, intestinal, and nervous tissues is in agreement with previously reported findings that both peptides are part of a single precursor molecule (preproglucagon).

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Authors and Affiliations

  1. Department of Anatomy, University of Heidelberg, Im Neuenheimer Feld 307, D-6900, Heidelberg, Germany

    Th. Kauth & J. Metz

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  1. Th. Kauth
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  2. J. Metz
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Supported by the DFG, SFB 90

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Kauth, T., Metz, J. Immunohistochemical localization of glucagon-like peptide 1. Histochemistry 86, 509–515 (1987). https://doi.org/10.1007/BF00500625

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  • DOI: https://doi.org/10.1007/BF00500625

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