Summary
The path of the transepithelial Na transport through the frog skin was investigated by the use of compartment analysis. Therefore tracer wash-out kinetics from the preloaded epithelium to both sides of the skin and time course of tracer uptake at the epithelial side were measured under control conditions and after alteration of transport parameter by amiloride, ouabain and incubation at 0°C. In all experiments the skins were short-circuited throughout and the SCC registered.
The results indicate that the major part of the Na involved in transport is already transported and located in an extracellular compartment, possibly the intercellular space between the epithelial cells. The decisive step of the transepithelial transport seems to be located at the outer epithelial border of the cell layer since entrance of Na into the skin is similarly altered by all inhibitors of transport.
Two additional compartments which are labelled from the epithelial side are detectable. The Na content of the compartment with the lowest exchange rate is increased by ouabain and 0° C but remains unchanged after amiloride. It might be located intracellularly but is not responsible for transepithelial transport. The actual transport pool must be very small.
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Nagel, W., Dörge, A., Moshagen, D. et al. Analysis of sodium compartments and fluxes in frog skin. Naunyn-Schmiedeberg's Arch. Pharmacol. 281, 281–294 (1974). https://doi.org/10.1007/BF00500597
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DOI: https://doi.org/10.1007/BF00500597