, Volume 92, Issue 4, pp 325–329 | Cite as

Proliferative activity of gastric and duodenal endocrine cells in the rat

  • F. J. G. M. Kubben
  • F. T. Bosman


The replicative activity and migration of gastrin, somatostatin and serotonin cells in rat stomach and doudenum was studied using combined immunocytochemistry and autoradiography after 3H thymidine pulse-labeling. Our results show that a small proportion of gastrin, somatostatin and serotonin immunoreactive cells displays proliferative activity. The overall labeling index ranged from 1.3% for gastric endocrine cells to 3.2% for duodenal endocrine cells.

In a pulse chase experiment, labeling indices of immunoreactive cells were estimated at several time intervals after 3H thymidine administration. Significant differences in labeling index were not found. Migration of 3H thymidine labeled endocrine cells towards the luminal surface was not found in the stomach nor in the doudenum.

It is concluded that 1) these endocrine cells have replicating activity; 2) the replicative activity of endocrine cells is higher in the duodenum than in the stomach; 3) the various cell types do not show significant differences in replicating activity and 4) endocrine cells did not seem to migrate to the luminal surface of the mucosa along with the other epithelial cells.


Serotonin Thymidine Proliferative Activity Label Index Endocrine Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Andrew A (1984) The development of the gastro-entero-pancreatic neuroendocrine system in birds. In: Falkmer S, Håkanson R, Sundler F (eds) Evolution and tumour pathology of the endocrine system. Elsevier, Amsterdam, pp 91–109Google Scholar
  2. Bosman FT (1984) Neuro-endocrine cells in non-neuroendocrine tumours. In: Falkmer S, Håkanson R, Sundler F (eds) Evolution and tumour pathology of the endocrine system. Elsevier, Amsterdam, pp 519–543Google Scholar
  3. Bosman FT, Louwerens JWK (1981) APUD-cells in teratomas. Am J Pathol 104:174–180Google Scholar
  4. Chang WWL, Leblond CP (1971) Renewal of the epithelium in the descending colon of the mouse. II. Renewal of argentaffin cells. Am J Anat 131:101–106Google Scholar
  5. Cheng H, Leblond CP (1974a) Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. III. Entero-endocrine cells. Am J Anat 141:503–509Google Scholar
  6. Cheng H, Leblond CP (1974b) Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. V. Unitarian theory of the origin of the four epithelial cell types. Am J Anat 141:537–549Google Scholar
  7. Deschner EE, Lipkin M (1966) An autoradiographic study of the renewal of argentaffin cells in the human rectal mucosa. Exp. Cell Res 43:661–665Google Scholar
  8. Fereira MN, Leblond CP (1971) Argentaffin and other “endocrine” cells of the small intestine in the adult mouse. II. Renewal. Am J Anat 131:331–352Google Scholar
  9. Fujimoto S, Hattori T, Kimoto K, Yashamita S, Fujika S, Kawai K (1980) Tritiated thymidine autoradiographic study on origin and renewal of gastrin cells in antral area of hamsters. Gastroenterology 79:785–790Google Scholar
  10. Graham RC, Karnovsky MC (1966) The early steps of absorption of injected horscradish peroxidase in the proximal tubules of the mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–298Google Scholar
  11. Grossmann MI (1981) General concepts. In: Bloom SR, Polak JM (eds) Gut hormones, 2nd ed. Churchill Livingstone, Edinburgh, pp 17–22Google Scholar
  12. Lehy T (1982) Self-replication of somatostatin cells in the antral mucosa of rodents. Cell Tissue Kinet 15:495–505Google Scholar
  13. Lehy T, Willems G (1975) Combined use of immunocytochemical and autoradiographic techniques for studying the renewal of gastrin cells. Biomed Express 23:443–446Google Scholar
  14. Lehy T, Willems G (1976) Population kinetics of antral gastrin cells in the mouse. Gastroenterology 71:614–619Google Scholar
  15. Nieuwenhuyzen-Kruseman AC, Knijnenburg G, Brutel de la Riviere G, Bosman FT (1978) Morphology and immunohistochemically defined endocrine function of pancreatic islet cell tumors. Histopathology 2:389–399Google Scholar
  16. Odartchenko N, Hedinger C, Ruzicka J, Weber E (1970) Cytokinetics of argentaffin cells in mouse intestinal mucosa. Virchows Arch B 6:132–136Google Scholar
  17. Patzelt V (1936) Der Darm. In: von Mollendorff W (ed) Handbuch der Mikroskopischen Anatomie des Menschen. Springer, Berlin Heidelberg New York, pp 111–120Google Scholar
  18. Pearse AGE (1969) The cytochemistry and ultrastructure of polypeptide hormone producing cells of the APUD series and the embryologic, physiologic and pathologic implications of the concept. J Histochem Cytochem 17:307–313Google Scholar
  19. Schutte B, Reijnders MMJ, Bosman FT, Blijham GH (1987) Studies with anti bromodeoxyuridine antibodies. II. Simultaneous detection of DNA synthesis and antigen expression by immunocytochemistry. J Histochem Cytochem 35:371–374Google Scholar
  20. Sidhu G (1979) The endocrine origin of digestive and respiratory tract APUD cells. Am J Pathol 96:5–20Google Scholar
  21. Sjölund K, Sanden G, Håkanson R, Sundler F (1983) Endocrine cells in the human intestine: An immunocytochemical study. Gastroenterology 85:1120–1130Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • F. J. G. M. Kubben
    • 1
  • F. T. Bosman
    • 1
  1. 1.Department of PathologyUniversity of Limburg, Medical SchoolMaastrichtThe Netherlands

Personalised recommendations