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Toxicity of a highly purified leucocidin from Pseudomonas aeruginosa in perfused rat livers

  • M. Frimmer
  • W. Scharmann
Article

Summary

Leucocidin, a toxic protein obtained from Pseudomonas aeruginosa, was tested in perfused rat livers. Doses of >4μg/g liver produced a heavy potasium loss and a massive decrease of the perfusion rate but only moderate swelling of the liver at 37°C. All effects depended on the dose. If the perfusions were carried out at 22 or 9°C neither K+-loss nor swelling, and only a moderate increase of the vascular resistance was observed. Swelling at 37°C was stronger at low initial perfusion rates (2.0 ml·min−1·g−1) than at higher ones (4.0 ml·min−1·g−1). The major effect of leucocidin could be prevented when the perfusion medium was changed 30 min after the addition of the toxin. Only small amounts of 125I-leucocidin were taken up by the liver tissue. The time course and the quantity of nearly all effects of leucocidin were quite different from those of phalloidin.

Key words

Leucocidin from Pseudomonas aeruginosa Perfused Rat Liver Potassium Loss Liver Swelling Vascular Resistance 

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References

  1. Callahan III, L. T.: Purification and characterization of Pseudomonas aeruginosa exotoxin. Infect. Immun. 9, 113–118 (1974)Google Scholar
  2. Frimmer, M.: The influence of physical conditions on swelling and K+-release in perfused rat livers poisoned with phalloidin. Naunyn-Schmiedeberg's Arch. Pharmacol. 275, 393–403 (1972a)Google Scholar
  3. Frimmer, M.: Temperature dependence of potassium depletion in the phalloidin poisoned perfused rat liver. Naunyn-Schmiedeberg's Arch. Pharmacol. 272, 354–357 (1972b)Google Scholar
  4. Frimmer, M., Gries, J., Hegner, D., Schnorr, B.: Untersuchungen zum Wirkungsmechanismus des Phalloidins. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 258, 197–214 (1967)Google Scholar
  5. Frimmer, M., Kroker, R.: Phalloidinantagonisten: 1. Mitteilung. Wirkung von Silybinderivaten an der isoliert perfundierten Rattenleber. Arzneimittel-Forsch. (Drug Res.) 25, 394–396 (1975)Google Scholar
  6. Gladstone, G. P., van Heyningen, W. E.: Staphylococcal leucocidins. Brit. J. exp. Path. 38, 123–137 (1957)Google Scholar
  7. Habermann, E.: Pharmakokinetische Besonderheiten des Tetanustoxins und ihre Beziehungen zur Pathogenese des lokalen bzw. generalisierten Tetanus. Naunyn-Schmiedebergs Arch. Pharmak. 267, 1–19 (1970)Google Scholar
  8. Liu, P. V.: Extracellular Toxins of Pseudomonas aeruginosa. J. infect. Dis. 130, 94–99 (1974)Google Scholar
  9. Liu, P. V., Yoshii, S., Hsieh, H.: Exotoxins of Pseudomonas aeruginosa. II. Concentration, purification and characterization of exotoxin A. J. infect. Dis. 128, 514–519 (1973)Google Scholar
  10. McConahey, P. J., Dixon, F. J.: A method of trace iodination of proteins for immunologic studies. Int. Arch. Allergy 29, 185–189 (1966)Google Scholar
  11. Puchinger, H., Wieland, Th.: Suche nach einem Metaboliten bei Vergiftung mit Desmethylphalloin. Europ. J. Biochem. 11, 1–6 (1969)Google Scholar
  12. Scharmann, W.: Leukozidin von Pseudomonas aeruginosa. Habilitationsschrift, Justus Liebig-Universität Gießen, FB 18 (1975)Google Scholar
  13. Sensakovic, J. W., Bartell, P. F.: The slime of Pseudomonas aeruginosa: a biological characterization and possible role in experimental infection. J. infect. Dis. 129, 101–109 (1974)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • M. Frimmer
    • 1
    • 2
  • W. Scharmann
    • 1
    • 2
  1. 1.Institut für Pharmakologie und Toxikologie im Fachbereich Vet.-MedGießenGermany
  2. 2.Institut für Bakteriologie und Immunologie im Fachbereich Veterinärmedizin der Justus Liebig-UniversitätGießenGermany

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