Toxicity of a highly purified leucocidin from Pseudomonas aeruginosa in perfused rat livers

  • M. Frimmer
  • W. Scharmann


Leucocidin, a toxic protein obtained from Pseudomonas aeruginosa, was tested in perfused rat livers. Doses of >4μg/g liver produced a heavy potasium loss and a massive decrease of the perfusion rate but only moderate swelling of the liver at 37°C. All effects depended on the dose. If the perfusions were carried out at 22 or 9°C neither K+-loss nor swelling, and only a moderate increase of the vascular resistance was observed. Swelling at 37°C was stronger at low initial perfusion rates (2.0 ml·min−1·g−1) than at higher ones (4.0 ml·min−1·g−1). The major effect of leucocidin could be prevented when the perfusion medium was changed 30 min after the addition of the toxin. Only small amounts of 125I-leucocidin were taken up by the liver tissue. The time course and the quantity of nearly all effects of leucocidin were quite different from those of phalloidin.

Key words

Leucocidin from Pseudomonas aeruginosa Perfused Rat Liver Potassium Loss Liver Swelling Vascular Resistance 


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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • M. Frimmer
    • 1
    • 2
  • W. Scharmann
    • 1
    • 2
  1. 1.Institut für Pharmakologie und Toxikologie im Fachbereich Vet.-MedGießenGermany
  2. 2.Institut für Bakteriologie und Immunologie im Fachbereich Veterinärmedizin der Justus Liebig-UniversitätGießenGermany

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