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Sensitization of adipose tissue to insulin by rat serum

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Summary

The oxidation of the C-1 atom of glucose through the pentose shunt of adipose tissue, under maximal stimulation by insulin, was enhanced by rat serum. The material responsible for the increase of the efficacy of insulin by serum was ultrafiltrable and partly dialysable. At submaximal concentrations of insulin, the serum-induced enhancement of responses to insulin resulted in an increase of the relative potency of insulin (potentiation).

Sodium acetate modified the log dose-response curve of insulin in the same way as serum did. Furthermore, acetate was active at low concentrations, suggesting that serum acetate might contribute to the sensitization to insulin by serum. In the presence of glucose and insulin, acetate is known to activate the rate of synthesis of fatty acids, which, in turn, determines the activation of the pentose shunt. Other constituents of serum known to influence the metabolism of adipose tissue in the same way as acetate may be expected to contribute to the sensitization to insulin by serum.

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Authors and Affiliations

  1. Institute de Pharmacologie de l'Université de Lausanne, Lausanne, Switzerland

    R. Guidoux, Gisela Möhren & G. Peters

Authors
  1. R. Guidoux
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  2. Gisela Möhren
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  3. G. Peters
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Additional information

Preliminary communication: Guidoux and Peters, 1970; Guidoux et al. 1972.

Supported by Fonds National Suisse de la Recherche Scientifique, Grants No. 5316, 3.370.70 and 3.736.72.

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Guidoux, R., Möhren, G. & Peters, G. Sensitization of adipose tissue to insulin by rat serum. Naunyn-Schmiedeberg's Arch. Pharmacol. 283, 37–52 (1974). https://doi.org/10.1007/BF00500144

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  • DOI: https://doi.org/10.1007/BF00500144

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