Abstract
Starch gel electrophoresis and histochemical staining with l-leucyl-l-tyrosine have revealed genetic variation for dipeptidase in Rattus norvegicus. The tissue distribution, substrate specificity, and heterozygous expression as a monmeric protein suggest homology of the variant peptidase to human PEP-C and mouse Pep-3 (Dip-1). We propose Peptidase-3 (Pep-3) as a name for this autosomal locus in the rat. The allele responsible for slower (less anodal) electrophoretic migration is designated Pep-3 a and is characteristic of strain ACI/Pit. A faster (more anodal) electrophoretic mobility is the product of the Pep-3 b allele in strain F344/Pit. Twenty-five additional inbred strains carry Pep-3 a and 16 others carry Pep-3 b. Wild rats trapped in Pittsburgh were polymorphic for this locus. Alleles at Pep-3 segregated independently of c (linkage group I), a (linkage group IV), RT2 and Es-1 (linkage group V), h (linkage group VI), and RTI (linkage group VIII).
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This work was supported by grants from the National Foundation/March of Dimes and the National Institutes of Health (CA 25038). Dr. Cramer is a recipient of the Research Career Development Award AI00314.
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Womack, J.E., Cramer, D.V. Peptidase-3 (Pep-3), dipeptidase variant in the rat homologous to mouse Pep-3 (Dip-1) and human PEP-C. Biochem Genet 18, 1019–1026 (1980). https://doi.org/10.1007/BF00500132
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DOI: https://doi.org/10.1007/BF00500132