Biochemical Genetics

, Volume 18, Issue 9–10, pp 869–877 | Cite as

Analysis of disproportionate replication of ribosomal DNA in Drosophila melanogaster by a microhybridization method

  • Barry Yedvobnick
  • Hallie M. Krider
  • F. Lee Dutton


A microhybridization technique is described which requires only 1% of the starting material normally needed for filter-bound methods. Employing this technique, we have investigated the disproportionate replication (compensation) of ribosomal DNA in larval and adult stages of two strains of Drosophila melanogaster. Both stages of the Oregon R strain demonstrate XO compensation while neither stage of Canton S shows a significant elevation of ribosomal DNA content in XOs. It is demonstrated that the lack of disproportionate replication in the latter strain does not result from the absence of the genetic site cr+ which normally controls this process.

Key words

disproportionate replication Drosophila rDNA microhybridization 


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  1. Dawid, I. B., Wellauer, P. K., and Long, E. O. (1978). Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments. J. Mol. Biol. 126749.Google Scholar
  2. Durica, D. S., and Krider, H. M. (1977). Studies on the ribosomal RNA cistrons in interspecific Drosophila hybrids. I. Nucleolar dominance. Dev. Biol. 5962.Google Scholar
  3. Gall, J. G. (1969). The genes for ribosomal RNA during oogenesis. Genetics 61121 (Suppl. 1).Google Scholar
  4. Hennig, W., and Meer, B. (1971). Reduced polyteny of ribosomal RNA cistrons in giant chromosomes of Drosophila hydei. Nature New Biol. 23370.Google Scholar
  5. Kissane, H. M., and Robins, E. (1958). The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. J. Biol. Chem. 233184.Google Scholar
  6. Krider, H. M., Yedvobnick, B., and Levine, B. I. (1979). The effect of abo phenotypic expression on ribosomal DNA instabilities in Drosophila melanogaster. Genetics 92879.Google Scholar
  7. Mohan, J. (1976). Ribosomal DNA and its expression in Drosophila melanogaster during growth and development. Mol. Gen. Genet. 147217.Google Scholar
  8. Procunier, J. D., and Tartof, K. D. (1978). A genetic locus having trans and contiguous cis functions that control the disproportionate replication of ribosomal RNA genes in Drosophila melanogaster. Genetics 8867.Google Scholar
  9. Reeder, R. H. (1974). Ribosomes from eukaryotes: Genetics. In Ribosomes Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., pp. 489–518.Google Scholar
  10. Ritossa, F. M. (1976). The bobbed locus. In The Genetics and Biology of Drosophila Academic Press, New York, pp. 801–846.Google Scholar
  11. Ritossa, F. M., and Spiegelman, S. (1965). Localization of DNA complementary to ribosomal RNA in the nucleolus organizer region of Drosophila melanogaster. Proc. Natl. Acad. Sci. 53737.Google Scholar
  12. Sibitani, A. (1971). Difference in the proportion of the DNA specific to ribosomal RNA between adults and larvae of Drosophila melanogaster. Mol. Gen. Genet. 114177.Google Scholar
  13. Spear, B. B., and Gall, J. G. (1973). Independent control of ribosomal gene replication in polytene chromosomes of Drosophila melanogaster. Proc. Natl. Acad. Sci. 701359.Google Scholar
  14. Tartof, K. D. (1971). Increasing the multiplicity of ribosomal RNA genes in Drosophila melanogaster. Science 171294.Google Scholar
  15. Wensink, P. C., Finnegan, D. J., Donelson, J. E., and Hogness, D. S. (1974). A system for mapping DNA sequences in the chromosomes of Drosophila melanogaster. Cell 3315.Google Scholar

Copyright information

© Plenum Publishing Corporation 1980

Authors and Affiliations

  • Barry Yedvobnick
    • 1
  • Hallie M. Krider
    • 1
  • F. Lee Dutton
    • 1
  1. 1.Biological Sciences Group, Genetics and Cell Biology SectionThe University of ConnecticutStorrs

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