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Ultrasound sensitive enzyme-membrane

  • Yoshio Ishimori
  • Isao Karube
  • Shuichi Suzuki
Biotechnology

Summary

Glucose oxidase (GOD) was immobilized on an acetylcellulose membrane filter (pore size: 0.45 μm) with dipalmitoyl phosphatidyl-choline (DPPC). The activity of the GOD-DPPC membrane appeared during ultrasonic irradiation while the GOD activity disappeared when the irradiation was stopped. The apparent GOD activity (the value of the output current decrease of an oxygen electrode in the reaction mixture) gradually increased with increasing ultrasonic power below 3 W. On the other hand, it decreased with the increase in ultrasonic power over 4 W because dissolved oxygen in the reaction mixture in creased as ultrasonic cavitation was generated. The response of the membrane decreased with increasing ultrasonic frequency and the response of the membrane to ultrasound waves (20 KHz, 3 W) was reproducible. The membrane was stable for at least 2 months at 5°C. We conclude that the GOD-DPPC membrane can be regarded as a ultrasound sensitive membrane.

Keywords

Pore Size Dissolve Oxygen Cavitation Membrane Filter Glucose Oxidase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Gregoriadis G (1976) Medical applications of liposome-entrapped enzymes. In: Mosbach K (ed) Methods in enzymology, vol 44. Academic Press New York, pp 698–709Google Scholar
  2. Ishimori Y, Karube I, Szuki S (1981a) Mechanical control of the activity of glucose oxidase immobilized on porous polyvinylchloride membrane. Biotechnol Bioeng 23:1267–1274Google Scholar
  3. Ishimori Y, Karube I, Suzuki S (1981b) Stress sensitive membrane. Eur J Appl Microbiol Biotechnol 13:19–23Google Scholar
  4. Ishimori Y, Karube I, Suzuki S (1981c) Continuous production of glucose oxidase with Aspergillus sp. under ultrasound waves. Enzyme Microbial Technol (in press)Google Scholar
  5. Karube I, Nakamoto Y, Namba K, Suzuki S (1976) Photocontrol of urease. collagen membrane activity. Biochim Biophys Acta 429:975–981Google Scholar
  6. Klibanov AM, Samokhin GP, Martinek K, Berezin I (1977) A new mechanochemical method of enzyme immobilization. Biotechnol Bioeng 19:211–218Google Scholar
  7. Matsuoka H, Suzuki S, Aizawa M, Kimura Y, Ikegami A (1981) Enzyme switch using purple membrane-enzyme vesicle. J Appl Biochem (in press)Google Scholar
  8. Mosbach K (ed) (1976) Immobilization techniques. In: Methods in enzymology, vol 44. Academic Press, New York, pp 11–332Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Yoshio Ishimori
    • 1
  • Isao Karube
    • 1
  • Shuichi Suzuki
    • 1
  1. 1.Research Laboratory of Resources UtilizationTokyo Institute of TechnologyYokohamaJapan

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