Ultrasound sensitive enzyme-membrane
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Glucose oxidase (GOD) was immobilized on an acetylcellulose membrane filter (pore size: 0.45 μm) with dipalmitoyl phosphatidyl-choline (DPPC). The activity of the GOD-DPPC membrane appeared during ultrasonic irradiation while the GOD activity disappeared when the irradiation was stopped. The apparent GOD activity (the value of the output current decrease of an oxygen electrode in the reaction mixture) gradually increased with increasing ultrasonic power below 3 W. On the other hand, it decreased with the increase in ultrasonic power over 4 W because dissolved oxygen in the reaction mixture in creased as ultrasonic cavitation was generated. The response of the membrane decreased with increasing ultrasonic frequency and the response of the membrane to ultrasound waves (20 KHz, 3 W) was reproducible. The membrane was stable for at least 2 months at 5°C. We conclude that the GOD-DPPC membrane can be regarded as a ultrasound sensitive membrane.
KeywordsPore Size Dissolve Oxygen Cavitation Membrane Filter Glucose Oxidase
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