Recovery of invertase and laminarinases from industrial waste broths of baker's yeast

  • T. G. Villa
  • H. J. Phaff
Industrial Microbiology


A procedure is described for the recovery of invertase and three laminarinases from commercial waste broths of baker's yeast. The procedure includes adsorption steps on DEAE-Sephadex A50, on concanavalin A-Sepharose 4B, and an additional gel exclusion chromatography step on Sephacryl S-200. The isolated laminarinases were: laminarinase I (exo-β-D-glucanase), laminarinase II [endo-β-(1-3)-D-glucanase], and laminarinase III (exo-β-D-glucanase). The efficiency of the main concentration step (DEAE-Sephadex A50) depended on the predialysis of the crude broth. Thus, the recovery of invertase and laminarinase from nondialyzed samples was 7% and 16%, respectively. These values were increased to 62% and 100%, respectively, when the samples were previously dialyzed.


Chromatography Industrial Waste Concanavalin Chromatography Step Concentration Step 
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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • T. G. Villa
    • 1
  • H. J. Phaff
    • 1
  1. 1.Department of Food Science and TechnologyUniversity of CaliforniaDavisUSA

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