Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 330, Issue 2, pp 136–141 | Cite as

Leukotrienes C4, D4 and E4 cause widespread and extensive plasma extravasation in the guinea pig

  • X.-Y. Hua
  • S.-E. Dahlén
  • J. M. Lundberg
  • S. Hammarström
  • P. Hedqvist
Article

Summary

  1. 1.

    Intravenous injection of leukotriene C4 (1 nmol × kg−1) caused substantial plasma exudation in anesthetized guinea pigs, as evidenced by marked hemoconcentration 15% in 5 min) and significant extravasation of Evans blue.

     
  2. 2.

    Fluorometric quantitation of Evans blue content in 38 selected tissues documented that leukotriene C4 caused significant plasma extravasation throughout the body, except for the brain, stomach, duodenum, colon and gonads.

     
  3. 3.

    In particular, the respiratory and the uro-genital tracts, but also the conjunctiva, the esophagus, the bile ducts and the umbilical ligaments, were very sensitive to the edemapromoting effect of leukotriene C4.

     
  4. 4.

    Intravenous injection of leukotriene D4 (1 nmol × kg −1) or E4 (5 nmol × kg−1) evoked plasma extravasation with a distribution and magnitude that was similar to that induced by leukotriene C4.

     
  5. 5.

    It is concluded that the three major constituents of slow reacting substance of anaphylaxis (SRS-A), leukotrienes C4, D4 and E4, cause a generalized and extensive plasma exudation that is consistent with the proposal that these leukotrienes are important mediators of inflammation.

     

Key words

Leukotriene C4 Leukotriene D4 Leukotriene E4 Plasma extravasation Inflammation 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • X.-Y. Hua
    • 1
  • S.-E. Dahlén
    • 2
  • J. M. Lundberg
    • 1
  • S. Hammarström
    • 3
  • P. Hedqvist
    • 2
    • 4
  1. 1.Department of PharmacologyKarolinska InstitutetStockholmSweden
  2. 2.Department of PhysiologyKarolinska InstitutetStockholmSweden
  3. 3.Department of Physiological ChemistryKarolinska InstitutetStockholmSweden
  4. 4.National Institute of Environmental MedicineStockholmSweden

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