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Serial production of nucleoside-5′-triphosphates and uracil from 3′-mononucleotides by intact yeast cells

  • S. Watanabe
  • R. Kodaira
  • Y. Kaneko
Short Communication
  • 23 Downloads

Summary

Nucleoside-5′-triphosphates such as 5′-ATP and 5′-GTP can be produced efficiently and continuously from 3′-mononucleotides such as 3′-AMP and 3′-GMP by a series of processes consisting of two reaction phases using dried cells of Candida sp. N-25-2 (a hydrocarbon assimilating yeast). Moreover, incidentally to the 5′-triphosphates, free uracil is yielded almost stoichiometrically from 3′-CMP and 3′-UMP which, as is well known, are main concomitant products depolymerized from RNA. Uracil is then also available for many usage.

Keywords

Hydrocarbon Yeast Cell Candida Triphosphate Uracil 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Shirota S, Watanabe S, Takeda I (1971) Effects of Pyrophosphate on Formation of Nucleotide Derivatives. Agr Biol Chem 35 325–332Google Scholar
  2. Watanabe S, Takeda I (1972) Effects of Cultural conditions on Mononucleotide Phosphorylating Activity of Yeast Cells. Agr Biol Chem 36 2263–2273Google Scholar
  3. Watanabe S, Osawa T, Yamamoto S (1968) Excretion of Nucleotides from Yeast Cells and Actions of Surfactant. J Ferment Technol 46 538–544Google Scholar
  4. Watanabe S, Kitajima N, Shirota S, Takeda I (1981) Effects of Several Factors on CDP-choline Production by Yeast. J Ferment Technol 59 197–201Google Scholar
  5. Allen R J L (1940) The Estimation of Phosphorus. Biochem J 34 858–865Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • S. Watanabe
    • 1
  • R. Kodaira
    • 1
  • Y. Kaneko
    • 2
  1. 1.Research LaboratoryAsahi Chemical Industry, Co., Ltd.Tokyo
  2. 2.Department of Agricultural ChemistryNagoya UniversityNagoya, AichiJapan

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