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Cell recovery by continuous flotation

  • Biotechnology
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European journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

Cell recovery by means of continuous flotation of the Hansenula polymorpha cultivation medium without additives was investigated as a function of the cultivation conditions as well as of the flotation equipment construction and flotation operational parameters. The cell enrichment and separation is improved at high liquid residence times, high aeration rates, small bubble sizes, increasing height of the aerated column, and diameter of the foam column. Increasing cell age and cultivation with nitrogen limitation reduce the cell separation.

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Abbreviations

CP :

cell mass concentration in medium g·l−1

CR :

cell mass concentration in residue g·l−1

CS :

cell mass concentration in foam liquid g·l−1

V :

equilibrium foam volume cm3

V:

gas flow rate through the aerated liquid column cm3·s−1

VF :

feed rate to the flotation column ml/min

Σ 1 :

V S/V foaminess s

τ:

mean liquid residence time in the column s

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Authors and Affiliations

  1. Institut für Technische Chemie, Universität Hannover, Callinstraße 46, 3000, Hannover, Federal Republic of Germany

    Holger Viehweg & Karl Schügerl

Authors
  1. Holger Viehweg
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  2. Karl Schügerl
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Viehweg, H., Schügerl, K. Cell recovery by continuous flotation. European J. Appl. Microbiol. Biotechnol. 17, 96–102 (1983). https://doi.org/10.1007/BF00499858

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  • DOI: https://doi.org/10.1007/BF00499858

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