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Qualitative yeast enzyme analysis by electrophoresis

  • Biotechnology
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European journal of applied microbiology and biotechnology Aims and scope Submit manuscript

Summary

Baker's yeast (Saccharomyces cerevisiae) was cultivated under different intensities of aeration on glucose and on ethanol. Seventeen enzymes of the Embden-Meyerhof pathway and the TCA cycle or related reactions were then assayed by starch gel electrophoresis. There were both qualitative and quantitative differences in many enzymes, most notably in glyceraldehyde-3-phosphate dehydrogenase, alcohol dehydrogenase, isocitrate dehydrogenase, malate dehydrogenase, and fumarase. Enzyme electrophoresis seems to offer a promising method for rapidly obtaining information about many yeast enzymes from a large number of samples.

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References

  • Bartholmes P, Jaenicke R (1978) Reassociation and reactivation of yeast glyceraldehyde-3-phosphate dehydrogenase after dissociation in the presence of ATP. Eur J Biochem 87:563–567

    Google Scholar 

  • Bauchop T, Elsden SR (1960) The growth of micro-organisms in relation to their energy supply. J Gen Microbiol 23:457–469

    Google Scholar 

  • Colowick SP (1973) The hexokinases. In: Boyer PD (ed) The enzymes, 3rd edn, vol. 9B. Academic Press, New York, pp 1–48

    Google Scholar 

  • Hägele E, Neeff J, Mecke D (1978) The malate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Eur J Biochem 83:67–76

    Google Scholar 

  • Machado A, Nuñez de Castro I, Mayor F (1975) Isocitrate dehydrogenase and oxoglutarate dehydrogenase activities of baker's yeast grown in a variety of hypoxic conditions. Mol Cell Biochem 6:93–100

    Google Scholar 

  • Maitra PK (1970) A glucokinase from Saccharomyces cerevisiae. J Biol Chem 245: 2423–2431

    Google Scholar 

  • Nei M (1975) Molecular population genetics and evolution. North Holland, Amsterdam

    Google Scholar 

  • Oura E (1972) The effect of aeration on the growth energetics and biochemical composition of baker's yeast. Ph.D. Thesis, University of Helsinki, Finland

    Google Scholar 

  • Oura E (1974a) Effect of aeration intensity on the biochemical composition of baker's yeast. I. Factors affecting the type of metabolism. Biotechnol Bioeng 16:1197–1212

    Google Scholar 

  • Oura E (1974b) Effect of aeration on the biochemical composition of baker's yeast. II. Activities of the oxidative enzymes. Biotechnol Bioeng 16:1213–1225

    Google Scholar 

  • Oura E (1976) The effect of aeration intensity on the biochemical composition of baker's yeast: Activities of enzymes of the glycolytic and pentose phosphate pathways. Biotechnol Bioeng 18:415–420

    Google Scholar 

  • Oura E (1977) Some characteristic features of the anaerobic metabolism of yeast. EUCHEM Conference on Metabolic Reactions in the Yeast Cell in Anaerobic and Aerobic Conditions, Helsinki, pp 23–25

  • Oura E, Suomalainen H (1967) Free nucleotides in resting and metabolizing baker's yeast. J Inst Brew London 73:370–376

    Google Scholar 

  • Poulik MD (1957) Starch gel electrophoresis in a discontinuous system of buffers. Nature (London) 180:1477–1479

    Google Scholar 

  • Rijn J van, Wijk R van (1972) Differential sensitivities of the two malate dehydrogenases and the maltose permease to the effect of glucose in Saccharomyces carlsbergensis. J Bacteriol 110:477–484

    Google Scholar 

  • Saura A, Lokki J, Lokki M-L, Oura E, Suomalainen H (1977) A rapid method for the qualitative analysis of enzymes in yeast. EUCHEM Conference on Metabolic Reactions in the Yeast Cell in Anaerobic Conditions, Helsinki, pp 57–59

  • Scandalios JG (1974) Isoenzymes in development and differentiation. Annu Rev Plant Physiol 25:225–258

    Google Scholar 

  • Schatzmann H, Fiechter A (1974) Anaerobic chemostat-Kultur von Bäckerhefe. Chem Ing Tech MS 122/74

  • Shaw CR, Prasad R (1970) Starch gel electrophoresis of enzymes: a compilation of recipes. Biochem Genet 4:297–320

    Google Scholar 

  • Strobel R, Wöhrmann K (1978) The selective importance of allozymes in yeast populations. Egypt J Genet Cytol 7:108–122

    Google Scholar 

  • Suomalainen H (1963) Changes in the cell constitution of baker's yeast in changing growth conditions. Proc Symp Chem Biochem Fungi and Yeasts, Dublin. Pure Appl Chem 7:639–654

    Google Scholar 

  • Wöhrmann K, Lange P, Strobel R (1974) Populationsgenetische Untersuchungen am Saccharomyces cerevisiae. I. Die Hefe als populationsgenetisches Objekt. Theor Appl Genet 44:1–6

    Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, University of Helsinki, P. Rautatiekatu 13, SF-00100, Helsinki 10, Finland

    Anssi Saura & Juhani Lokki

  2. Research Laboratories of the State Alcohol Monopoly (Alko), Box 350, SF-00101, Helsinki 10, Finland

    Erkki Oura & Heikki Suomalainen

Authors
  1. Anssi Saura
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  2. Juhani Lokki
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  3. Erkki Oura
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  4. Heikki Suomalainen
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Saura, A., Lokki, J., Oura, E. et al. Qualitative yeast enzyme analysis by electrophoresis. European J. Appl. Microbiol. Biotechnol. 7, 355–364 (1979). https://doi.org/10.1007/BF00499850

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  • DOI: https://doi.org/10.1007/BF00499850

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