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Foam behavior of biological media

IV. Hansenula polymorpha cultivation foams
  • H. Buchholz
  • K. Kalischewski
  • K. Schügerl
Biotechnology

Summary

The surface tension σ and foaminess Σ of (a) unlimited, (b) substrate limited, and (c) oxygen transfer limited growth media of Hansenula polymorpha were measured using methanol, ethanol or glucose as a substrate.

The time dependence of σ can be described by the Avrami-Überreiter relationship: log (2.3 log V)=n log t+log b, where V = (σO − σeq/(σt − σeq, and σO, σt and σeq are σ at tM=0, tM=t and tM→∞ (equilibrium value).

The constants n and b are functions of the fermentation time tF as long as the growth is unlimited but they are constant in the state of limited growth. With glucose substrate, the foaminess Σ can be presented as a definite function of the time, tDG, which is necessary to attain σeq. With alcohol as a substrate no definite Σ (tDG) function was found.

Keywords

Oxygen Glucose Alcohol Methanol Fermentation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Symbols

b

constant in Eq. (1)

n

constant in Eq. (1)

S

substrate concentration

T

temperature

tM

time h (measured from the beginning of the determination of the surface tension σ)

tF

cultivation time h (measured from the time of inoculation)

tDG

time (min) necessary to attain the equilibrium surface tension σ)

X

dry biomass concentration (gl−1)

V

O − σeq)/(σt − σeq)

VS

equilibrium volume of the foam (cm3)

VG

volumetric gas flow rate during the estimation of Σ (cm3 s−1)

vvm

volumetric gas flow rate with regard to the volume of the medium (min−1)

wSG

superficial gas velocity (cm s−1)

μm

maximum specific growth rate (h−1)

Σ

VS/VG foaminess (s)

σ

surface tension, mMm−1 (milli Newton m−1)

σO

σ at tM=0

σeq

equilibrium surface tension (σ at tM→∞)

σt

σ at tM=t

HP

probes from Hansenula polymorpha cultivation

NLG

non limited growth

OTLG

oxygen transfer limited growth

SLG

substrate limited growth

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References

  1. Bikerman JJ (1938) Trans Faraday Soc 34:634Google Scholar
  2. Bumbullis W, Kalischewski K, Schügerl K (1979a) Part II (Salt effects). Eur J Appl Microbiol Biotechnol 7:147–154Google Scholar
  3. Bumbullis W, Schügerl K (1979b) Part V (Alcohol effects). Eur J Appl Microbiol Biotechnol (in press)Google Scholar
  4. Davies IT, Rideal EK (1963). Interfacial Phenomena. Academic Press, New York, p. 410Google Scholar
  5. Harkins WD, Jordan HF (1930) J Am Chem Soc 52:1751Google Scholar
  6. Kalischewski K, Bumbullis W, Schürgel K (1979) Part I (Protein foams). Eur J Appl Microbiol Biotechnol 7:21–31Google Scholar
  7. König B, Kalischewski K, Schügerl K (1979) III (Penicillium chrysogenum). Eur J Appl Microbiol Biotechnol 7:251–258Google Scholar
  8. Überreiter K, Morimoto S, Steulmann R (1974) Coll Polym Sci 252:273Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • H. Buchholz
    • 1
  • K. Kalischewski
    • 1
  • K. Schügerl
    • 1
  1. 1.Institut für Technische Chemie der Universität HannoverHannover 1Federal Republic of Germany

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