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A method for the fine-structural demonstration of lectin receptors

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Summary

A new two-step method using an Fc-fragment/férritin conjugate as a marker for the visualization of lectin-binding sites on neuronal and other cell membranes is described. In this study of rat synaptosomes, three lectins were tested: concanavalin A, mistletoe lectin I and wheat germ agglutinin. The specificity of the method was proved by control experiments.

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Authors and Affiliations

  1. Department of Anatomy, Histology and Embryology, Medical Academy, BG-1431, Sofia, Bulgaria

    K. Ichev & W. Ovtscharoff

  2. Staatliches Institut für Immunpräparate und Nährmedien, Klement-Gottwald-Allee 371-321, DDR-1120, Berlin, German Democratic Republic

    H. Franz & J. Mohr

Authors
  1. K. Ichev
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  2. W. Ovtscharoff
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  3. H. Franz
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  4. J. Mohr
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Ichev, K., Ovtscharoff, W., Franz, H. et al. A method for the fine-structural demonstration of lectin receptors. Histochemistry 84, 187–190 (1986). https://doi.org/10.1007/BF00499831

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  • DOI: https://doi.org/10.1007/BF00499831

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