, Volume 84, Issue 2, pp 161–168 | Cite as

Vitamin-D-dependent calcium-binding protein (CaBP-9K) in rat growth cartilage

  • N. Balmain
  • E. Tisserand-Jochem
  • M. Thomasset
  • P. Cuisinier-Gleizes
  • H. Mathieu


The presence of vitamin-D-dependent calcium-binding protein (CaBP-9K) in tibial growth-plate cartilage was immunohistochemically demonstrated using a specific antibody to rat duodenal CaBP-9K. The protein was found to be mainly localized in the cytoplasm of maturing chondrocytes. In hypertrophic chondrocytes, CaBP-9K concentrations decreased, and the protein was found in the cytoplasmic processes. No CaBP-specific immunoreactivity was seen in the hypertrophic chondrocytes of the lower calcified hypertrophic zone; in contrast, the protein was found in the extracellular lateral edges of longitudinal septa, i.e. where matrix vesicles are preferentially localized and where cartilage mineralization is initiated. These findings suggest that vitamin D has a direct function in this tissue. It also seems likely that CaBP-9K is an indicator of chondrocyte maturation, and that it is involved in the matrix vesicle-associated process of cartilage calcification.


Public Health Specific Antibody Direct Function Lateral Edge Cytoplasmic Process 
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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • N. Balmain
    • 1
  • E. Tisserand-Jochem
    • 2
  • M. Thomasset
    • 1
  • P. Cuisinier-Gleizes
    • 1
  • H. Mathieu
    • 1
  1. 1.INSERM Unité 120Le VésinetFrance
  2. 2.Service d'immuno-hématologie et d'immuno-pathologieInstitut PasteurParis Cédex 15France

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