, Volume 43, Issue 3, pp 275–282 | Cite as

Electron microscopic demonstration of cell surface carbohydrates by means of peroxidase and ferritin complexes of the Lens culinaris lection

  • J. Roth
  • K. Thoss
  • M. Wagner
  • H. W. Meyer


The use of Lens culinaris lectin for electron microscopic detection of D-mannose, D-glucose and N-acetyl-D-glucosamine like sites on tumor cells, erythrocytes, erythrocyte ghosts, cultured rat liver cells and various tissues of mice is demonstrated. In addition to Lens culinaris lectin-peroxidase reaction (LcL-po reaction) the preparation of active Lens culinaris lectin-ferritin conjugate are described and the specificity of cytochemical reactions are demonstrated. Furthermore experiments by immuno freeze-etching are reported for topological analysis of the lectin receptors.


Ferritin Topological Analysis Lens Culinaris Erythrocyte Ghost Microscopic Detection 
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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • J. Roth
    • 1
  • K. Thoss
    • 1
  • M. Wagner
    • 2
  • H. W. Meyer
    • 1
  1. 1.Institute of PathologyFriedrich-Schiller-UniversityJenaGerman Democratic Republic
  2. 2.Research Centre of Molecular Biology and Medicine, Central Institute of Microbiology and Experimental TherapyAcademy of Sciences of the GDRJenaGerman Democratic Republic

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