Summary
The synthesis of aldosterone was stimulated in vitro by ACTH, dibutyryl-cyclic AMP, potassium ions and angiotensin II. Aldosterone was determined by a method involving two thin layer chromatographies (TLC), a periodic acid oxidation step, and a final esterification with heptafluorobutyric anhydride to prepare the aldosterone-γ-lactone-heptafluorobutyrate for gas chromatographic quantification with electron-capture detection. The method is described in detail.
Spironolactone (6×10−5–1×10−4M) added to the incubation medium inhibited the synthesis of aldosterone in a dose-dependent manner, while the production of corticosterone was not affected.
It is concluded that spironolactone inhibits the conversion of corticosterone to aldosterone.
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Erbler, H.C. Stimulation of aldosterone production in vitro and its inhibition by spironolactone. Naunyn-Schmiedeberg's Arch. Pharmacol. 273, 366–375 (1972). https://doi.org/10.1007/BF00499670
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DOI: https://doi.org/10.1007/BF00499670