Histochemistry

, Volume 38, Issue 2, pp 143–153 | Cite as

Effect of specimen preparation on intracellular myocardial calcium

Electron microscopic X-ray microanalysis
  • R. Yarom
  • P. D. Peters
  • M. Scripps
  • S. Rogel
Article

Summary

Calcium concentration and intracellular localization were investigated using the electron microscopic X-ray analyser (EMMA-4) in preparations of dog myocardial tissues fixed either in glutaraldehyde, osmium tetroxide or potassium pyroantimonate plus osmium tetroxide.

Calcium could be detected with all the fixatives, but at varying efficiency. Glutaraldehyde fixation showed small detectable amounts of calcium and without special localization except in the nucleus. Osmium fixation showed much higher, but still poorly localized concentrations. The pyroantimonate technique produced the highest calcium concentrations and loci of statistically significant concentrations. Preparations stained with uranyl acetate or lead citrate showed well-visualized precipitates but no detectable calcium concentrations.

Keywords

Glutaraldehyde Uranyl Calcium Concentration Osmium Uranyl Acetate 

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • R. Yarom
    • 1
    • 2
    • 3
  • P. D. Peters
    • 1
    • 2
    • 3
  • M. Scripps
    • 1
    • 2
    • 3
  • S. Rogel
    • 1
    • 2
    • 3
  1. 1.Department of PathologyHebrew University-Hadassah Medical SchoolJerusalemIsrael
  2. 2.Cardiac Physiology LaboratoryHebrew University-Hadassah Medical SchoolJerusalemIsrael
  3. 3.the Cavendish LaboratoryCambridge UniversityCambridgeEngland

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