Neisseria gonorrhoeae RNA/DNA hybridization and culture for screening of gonococcal infections in a low-prevalence population
- 18 Downloads
Gonorrhea is still a major sexually transmitted disease (STD) worldwide. Its etiologic diagnosis is based on identification of the causative agent, Neisseria gonorrhoeae, by culture of genital secretions, which is often hampered by difficulties of sample collection and transport. Alternatively, nucleic acid hybridization techniques for routine diagnosis of N. gonorrhoeae appear to be useful by eliminating problems associated with bacterial viability, particularly for surveillance of low-prevalence populations. Our study among 1,508 outpatients undergoing routine examination for common STDs used RNA/DNA hybridization with a DNA probe specific for N. gonorrhoeae (Gen Probe Pace 2®) and classical culture. Of the 1,750 specimens tested, 12 were positive by DNA probe and culture. In 8 cases, only DNA probe was positive while culture was negative. In 3 of these discrepant cases clinical and epidemiological data suggested true N. gonorrhoeae infection. Thus, DNA probe assay for N. gonorrhoeae may greatly improve screening of N. gonorrhoeae among low-prevalence populations. However, culture remains mandatory for testing antimicrobial resistance of these highly communicable infectious agents.
Key wordsEpidemiology Identification Neisseria gonorrhoeae Nucleic acid detection Screening
Unable to display preview. Download preview PDF.
- 1.Hook EW, Holmes KK. Gonococcal infections. Ann Intern Med 1985; 102: 229–243.Google Scholar
- 2.Totten PA, Holmes KK, Handsfield HH, et al. DNA hybridization technique for the detection of Neisseria gonorrhoeae in men with urethritis. J Infect Dis 1983; 148: 462–471.Google Scholar
- 3.Lewis JS, Kranig-Brown D, Trainor DA. DNA probe confirmatory test for Neisseria gonorrhoeae. J Clin Microbiol 1990; 28: 2349–2350.Google Scholar
- 4.Roefaro-Franz M, Granato PA. Use of the Gen Probe PACE system for the detection of Neisseria gonorrhoeae in urogenital samples. Diagn Microbiol Infect Dis 1990; 13: 217–221.Google Scholar
- 5.Panke ES, Yang LI, Leist PA, et al. Comparison of Gen-Probe DNA probe test and culture for detection of Neisseria gonorrhoeae in endocervical specimens. J Clin Microbiol 1991; 29: 883–888.Google Scholar
- 6.Limberger RJ, Biega R, Evancoe A, et al. Evaluation of culture and the Gen-Probe PACE 2 assay for detection of Neisseria gonorrhoeae and Chlamydia trachomatis in endocervical specimens transported to a state health laboratory. J Clin Microbiol 1992; 30: 1162–1166.Google Scholar
- 7.Hale YM, Melton ME, Lewis JS, Willis DE. Evaluation of PACE 2 Neisseria gonorrhoeae assay by three public health laboratories. J Clin Microbiol 1993; 31: 451–453.Google Scholar
- 8.Vlaspolder F, Mutsaers JAEM, Blog F, Notowicz A. Value of a DNA Probe Assay (Gen-Probe) compared with that of culture for diagnosis of gonococcal infection. J Clin Microbiol 1993; 31: 107–110.Google Scholar
- 9.Stary A, Kopp W, Zahel B, et al. Comparison of DNAprobe test and culture for the detection of Neisseria gonorrhoeae in genital samples. Sex Transm Dis 1993; 20 No. 5: 243–247.Google Scholar