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Zur DNS-Synthesezeit in der menschlichen Epidermis

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Summary

  1. 1.

    DNA-synthesizing time of the human epidermis has been determined in vitro by a double labelling autoradiographic technique using 3H-thymidine. The DNA-synthesizing time is 5.9 hours at 37°C. Elevated temperatures seem to shorten this period.

  2. 2.

    In control studies the increasing labelling index after prolonged incubation with 3H-thymidine at 37° C has been evaluated and the increase of labelled cells per hour determined. In computing these data, the length of the DNA-synthesizing time was found to be in accordance with the previously found values.

  3. 3.

    From this the generation time of the basal cell layer has been calculated to be about 6–7 days. The need for exact knowledge on the length of the different cell cycle phases is emphasized.

Zusammenfassung

  1. 1.

    Unter in vitro-Bedingungen wurde die Dauer der DNS-Synthesezeit nach Doppelmarkierung mit 3H-Thymidin an menschlicher Haut autoradiographisch untersucht. Die Dauer der DNS-Synthesezeit beträgt etwa 5.9 Std bei 37°C. Bei steigender Temperatur wird eine Verkürzung dieser Zeit deutlich.

  2. 2.

    In Kontrolluntersuchungen wurden nach mehrstündiger Inkubation mit 3H-Thymidin die Zahl der 3H-markierten Zellen ausgezählt und der stündliche Zellzuwachs ermittelt. Die daraus gewonnenen Werte für die DNS-Synthesedauer entsprechen etwa den vorher gefundenen Werten.

  3. 3.

    Die durchschnittliche Verweildauer der Basalzelle im Str. germinativum beträgt unter normalen Bedingungen 6-7 Tage. Die Bedeutung genauerer Kenntnisse über die Phasendauer wird betont.

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Author information

Authors and Affiliations

  1. Dermatologische Klinik und Poliklinik der Philipps-Universität Marburg an der Lahn, Marburg an der Lahn, Deutschland

    E. Christophers

  2. Institut für Strahlenbiologie u. med. Isotopenanwendung der Philipps-Universität Marburg an der Lahn, Marburg an der Lahn, Deutschland

    E. Schaumlöffel

  3. Universitäts-Hautklinik, Deutschhausstr. 9, 3550, Marburg/Lahn

    E. Christophers

Authors
  1. E. Christophers
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  2. E. Schaumlöffel
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Durchgeführt mit Mitteln der Deutschen Forschungsgemeinschaft.

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Christophers, E., Schaumlöffel, E. Zur DNS-Synthesezeit in der menschlichen Epidermis. Arch. klin. exp. Derm. 228, 57–64 (1967). https://doi.org/10.1007/BF00499330

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  • DOI: https://doi.org/10.1007/BF00499330

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