Abstract
In vitro DNA:DNA hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid wheat species ancestral to the B genome of Triticum turgidum. 3H-T. turgidum DNA was hybridized to the unlabeled DNAs of T. urartu, T. speltoides, T. sharonensis, T. bicorne, T. longissimum, and T. searsii. 3H-Labeled DNAs of T. monococcum and a synthetic tetraploid AADD were hybridized with unlabeled DNAs of T. urartu and T. searsii to determine the relationship of the A genome of polyploid wheat and T. urartu. The heteroduplex thermal stabilities indicated that T. searsii was most closely related to the B genome of T. turgidum (AB) and that the genome of T. urartu and the A genome have a great deal of base-sequence homology. Thus, it appears that T. searsii is the B-genome donor to polyploid wheat or a major chromosome donor if the B genome is polyphyletic in origin.
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Published with the approval of the Director of The West Virginia Agricultural Experiment Station as Scientific Paper No. 1837.
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Nath, J., Hanzel, J.J., Thompson, J.P. et al. Additional evidence implicating Triticum searsii as the B-genome donor to wheat. Biochem Genet 22, 37–50 (1984). https://doi.org/10.1007/BF00499285
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DOI: https://doi.org/10.1007/BF00499285