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Histochemistry

, Volume 48, Issue 4, pp 269–281 | Cite as

Fluorogenic detection of primary amines in plant histochemistry with fluorescamine: A comparative study on the effects of coagulant and non-coagulant fixatives

  • Alessandro Bruni
  • Maria P. Fasulo
  • Barbara Tosi
  • Giuseppe Dall'Olio
  • Gian Luigi Vannini
Article

Summary

The new highly sensitive method of fluorescamine reaction for the topochemical detection of primary amino groups was studied as a substitude of ninhydrin-Schiff's reaction for the localisation of total proteins in plant tissues. The influence of various coagulant and non-coagulatn fixatives on the induction of fluorescamine fluorescence was examined: ethanol, formaldehyde gas and solution, glutaraldehyde, acrolein, osmium tetroxide, Bouin, Rossman, Clarke and Zenker's fluids and FMA were employed. It was found that the use of the fluorogenic method is conditioned by the fixative ability to keep the amino groups disposable and by its capability to reduce the natural autofluorescence of plant material. A detailed account of the fixation methodology demonstrated that non-coagulant acrolein and coagulant mercuric chloride are the most promising fixatives for the use of the fluorescamine reaction in plant histochemistry.

Keywords

Chloride Formaldehyde Plant Material Total Protein Plant Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Alessandro Bruni
    • 1
  • Maria P. Fasulo
    • 1
  • Barbara Tosi
    • 1
  • Giuseppe Dall'Olio
    • 1
  • Gian Luigi Vannini
    • 1
  1. 1.Intitute of BotanyUniversity of FerraraFerraraItaly

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