Skip to main content
SpringerLink
Log in

Inhibition by polyhalogenated hydrocarbons (PHHC) of ATPases in plasma membranes of parenchymal liver cells

  • Short Communication
  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Summary

Suspensions of freshly prepared plasma membranes from rat livers were equilibrated with defined mixtures of air with polyhalogenated hydrocarbons (PHHC) at 37°C. During the exposition enzymatic degradation of ATP was inhibited by carbon tetrachloride, chloroform and halothane in a dose-dependent manner. On a molar basis carbon tetrachloride was 5 times more effective than chloroform and halothane. The inhibition was partially reversible.

Diethyldithiocarbamate, an agent protecting animals against carbon tetrachloride, was ineffective in this assay.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Ames, B. N., Dubin, D. T.: The role of polyamines in the neutralization of bacteriophage desoxyribonucleic acid. J. biol. Chem. 235, 769–775 (1960)

    Google Scholar 

  • Bieri, V. G., Wallach, D. F. H., Lin, P. S.: Focal erythrocyte membrane pertubations caused by nitroxide lipid analogues. Proc. nat. Acad. Sci. (Wash.) 71, 4797–4801 (1974)

    Google Scholar 

  • Dorling, P. R., Le Page, R. N.: Studies of in vitro treatment of rat liver plasma membranes with carbon tetrachloride. Biochem. Pharmacol. 21, 2139–2141 (1972)

    Google Scholar 

  • Emmelot, P., Bos, C. J., Bennedetti, E. L., Rümke, Ph.: Studies on plasma membranes. I. Chemical composition and enzyme content of plasma membranes isolated from rat liver. Biochim. biophys. Acta (Amst.) 90, 126–145 (1964)

    Google Scholar 

  • Frimmer, M., Homann, J., Rufeger, U.: Hemmung akuter hepatotoxischer Wirkungen polyhalogenierter Kohlenwasserstoffe (PHKW) durch Diäthyldithiocarbamat (Dithiocarb). Anaesthesist 25, 89–96 (1976)

    Google Scholar 

  • Frimmer, M., Lutz, F.: Schwellung und Kaliumverlust bei perfundierten Lebern nach Einwirkung von dampfförmigem Tetrachlorkohlenstoff, Chioroform und Halothan auf das Perfusionsmedium. Anaesthesist 24, 112–117 (1975)

    Google Scholar 

  • Harper, A. E.: Glucose-6-phosphatase. In: Methoden der enzymatischen Analyse (H. U. Bergmeyer, ed.), pp. 782–792. Weinheim: Verlag Chemie 1962

    Google Scholar 

  • Hill, M. W.: The effect of anaesthetic-like molecules on the phase transition in smetic mesophases of dipalmitoyllecithin. Biochim. biophys. Acta 356, 117–124 (1974)

    Google Scholar 

  • Lange, P., Jung, F.: Die Verringerung der Lebertoxizität von Tetrachlorkohlenstoff durch Diäthyldithiokarbamat und 3-(O-Tolyl)-4-(nitro)-sydnon. Acta Biol. Med. Germ. 27, 425–434 (1971)

    Google Scholar 

  • Le Page, R. N., Dorling, P. R.: Plasma membranes in acute liver injury. Biochemical changes induced by carbon tetrachloride. Amer. J. exp. Biol. med. Sci. 49, 345–350 (1971)

    Google Scholar 

  • Lesko, L., Donlon, M., Marinetti, G. V., Hare, J. D.: A rapid method for the isolation of rat liver plasma membranes using an aqueous two-phase polymer system. Biochim. biophys. Acta (Amst.) 311, 173–179 (1973)

    Google Scholar 

  • Lin, P. S., Wallach, D. F. H., Mikkelsen, R. B., Schmidt-Ullrich, R.: Action of halothane on human erythrocytes, mechanism of cell lysis and production of sealed ghosts. Biochim. biophys. Acta (Amst.) 401, 73–82 (1975)

    Google Scholar 

  • Lutz, L. M., Glende, E. A., Jr., Recknagel, R. O.: Protection by diethyldithiocarbamate against carbon tetrachloride lethality in rats and against carbon tetrachloride induced lipid peroxidation in vitro. Biochem. Pharmacol. 22, 1729–1734 (1973)

    Google Scholar 

  • Metcalfe, J. C.: Magnetic resonance studies in membranes and lipids. In: The dynamic structure of cell membranes (D. F. H. Wallach and H. Fischer, eds.). Berlin-Heidelberg-New York: Springer 1971

    Google Scholar 

  • Michel, R. H., Hawthorne, J. N.: The site of diphosphoinositide synthesis in rat liver. Biochem. biophys. Res. Commun. 21, 333–338 (1965)

    Google Scholar 

  • Neville, D. M., Jr.: Isolation of an organ specific protein antigen from cell-surface membrane of rat liver. Biochim. biophys. Acta (Amst.) 154, 540–552 (1968)

    Google Scholar 

  • Weichselbaum, T. E.: Acurate and rapid method for determination of proteins in small amounts of blood serum and plasma. Amer. J. clin. Path., Techn. Sec. 10, 40–44 (1946)

    Google Scholar 

  • Yano, I., Masuda, Y., Kuchii, M., Yamamoto, H., Murano, T.: Studies on the function of cell membranes Electron microscopic observations on changes in ultrastructure of liver cell membranes in CCl4-treated rats. Jap. J. Pharmacol. 23, 645–652 (1973)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für Pharmakologie und Toxikologie im FB 18 der Justus Liebig-Universität Giessen, Frankfurter Strasse 107, D-6300, Giessen, Germany

    U. Rufeger & M. Frimmer

Authors
  1. U. Rufeger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Frimmer
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by the Deutsche Forschungsgemeinschaft.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rufeger, U., Frimmer, M. Inhibition by polyhalogenated hydrocarbons (PHHC) of ATPases in plasma membranes of parenchymal liver cells. Naunyn-Schmiedeberg's Arch. Pharmacol. 293, 187–190 (1976). https://doi.org/10.1007/BF00499226

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00499226

Key words

Search

Navigation