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Kinetische Analyse der Calcium-Kompartimente im Meerschweinchenherzen unter Kontrollbedingungen und Strophanthineinwirkung

Analysis of the calcium compartments in guinea pig hearts under control conditions and under the influence of ouabain

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Summary

A quantitative analysis of myocardial Ca-metabolism was carried out on isolated, isovolumetric (10 ml/min) perfused guinea pig hearts by combined determinations of the total Ca-content and kinetics of 45Ca-efflux (collecting period 60 min) (Fig. 1). The kinetics of 45Ca-uptake was estimated by extrapolating 45Ca-efflux curves of heart muscles isotopically loaded for different times (2, 5, 10, 30 60 min) to the end of the loading-period (Fig. 2).

From the specific activity it was possible to express the absolute Ca-content of the extrapolated compartments in μM Ca/g w.w. The amount of activity in cannules and coronary vessels was estimated by dextranblue (MW about 2 millions).

The results indicate the presence of three kinetically defined phases (K1, K2, K3) of calcium movements in guinea pig heart muscle. The content of the Ca compartments under equilibrium conditions (60 min 45Ca-loading period) was found to be: K1=0.29±0.05, K2=0.56±0.09, K3=0.30±0.04 μmoles/g w.w.

The half-time of Ca movement in these compartments for 45Ca efflux is for K1 15.5±1.0 sec, for K2 2.2±0.1 min, for K3 17.9±0.9 min, and for 45Ca uptake K1<2, K23.9± 1.11.0 , K39.2± 2.51.8 min.

Total Ca content has been found to be 1.67±0.06 μmoles/g w.w. Under control conditions about 65% of total heart muscle Ca was exchangeable. Under the influence of ouabain (1.5·10−7 M) Ca of the heart muscle exchanged to about 100%. This was caused by an increase in Ca content of compartment K3 to 0.46±0.06 μmoles/g w.w. and a significant decrease in total Ca-content to 1.32±0.07 μmoles/g w.w. The rate of 45Ca-exchange was not influenced by ouabain. The value of Ca-turnover for Ca-availability in contraction cycle and the mechanism of ouabain action are discussed.

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Authors and Affiliations

  1. Pharmakologisches Institut der Universität Mainz, Mainz, Deutschland

    W. Klaus & R. Krebs

  2. Abt. f. Biochemische Pharmakologie, Institut für Pharmakologie der Medizinischen Hochschule Hannover, Roderbruchstr. 101, D-3000, Hannover-Kleefeld, Federal Republic of Germany

    W. Klaus

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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

Ein Teil der Ergebnisse wurde anläßlich der Frühjahrstagung der Deutschen Pharmakologischen Gesellschaft im Mainz mitgeteilt (Krebs et al., 1970).

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Klaus, W., Krebs, R. Kinetische Analyse der Calcium-Kompartimente im Meerschweinchenherzen unter Kontrollbedingungen und Strophanthineinwirkung. Naunyn-Schmiedeberg's Arch. Pharmacol. 283, 277–292 (1974). https://doi.org/10.1007/BF00499188

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