Microphotometry of rat liver nuclear proteins
- 14 Downloads
One hour after partial hepatectomy some liver nuclei already have an increased nuclear protein content, after 15 hours all measured nuclei, and after 64 hours only a proportion of the measured nuclei.
A decrease of the Fast green histon content was found. This decrease occurred in the mayority of the nuclei 15 hours after partial hepatectomy. An increase in nuclear RNA was found for all measured nuclei at 15 hours, and for a proportion of the measured nuclei at 64 hours. Tetraploid and diploid nuclei behave identically.
The excess of nuclear protein after hepatectomy is resistent to elution with 0.14 M NaCl, which indicates a binding with chromosomal proteins or DNA.
The percentage of nuclear sap protein on the total nuclear protein content of tetraploid, diploid parenchymal nuclei and littoral cell nuclei, is equal. These nuclei differ in the amount of chromosomal bound proteins.
KeywordsPublic Health Protein Content Cell Nucleus Nuclear Protein Partial Hepatectomy
Unable to display preview. Download preview PDF.
- Auer, G.: Nuclear protein content and DNA-histone interaction. Exp. Cell Res. 75, 231–236 (1972)Google Scholar
- Bucher, N. L. R.: Experimental aspects of hepatic regeneration. New Engl. J. Med. 277, 686–696 (1968)Google Scholar
- Choe, B. K., Rose, N. R.: Synthesis of DNA binding protein during the cell cycle of W1-38 cells. Exp. Cell Res. 83, 271–280 (1974)Google Scholar
- Fabrikant, J. I.: The Kinetics of cellular proliferation in regenerating liver. J. Cell Biol. 36, 556–565 (1968)Google Scholar
- Goldstein, L.: On the question of protein synthesis by cell nuclei. Adv. Cell Biol. 1, 187–207 (1969)Google Scholar
- Harkness, R. O.: Liver regeneration. In: The Scientific basis of Medicine, Annual Reviews 236. London: Athlone Press of the University of London 1961Google Scholar
- James, J., Schopman, M., Delfgaauw, P.: The nuclear pattern of the parenchymal cells of the liver after partial hepatectomy. Exp. Cell Res. 42, 375–379 (1966)Google Scholar
- Kiefer, G., Kiefer, R., Sandritter, W.: Cytophotometric determination of nucleic acids in UV-light and after gallocyanine chromalum staining. Exp. Cell Res. 45, 247–249 (1966)Google Scholar
- Kostraba, N. C., Wang, T. Y.: Tissue variations of acidic nuclear proteins and their biosyntheses during liver regeneration. Int. J. Biochem. 1, 327–334 (1970)Google Scholar
- Laval, M., Bouteille, M.: Synthetic activity of isolated rat liver nuclei. Exp. Cell Res. 79, 391–403 (1973)Google Scholar
- Pogo, B. G. T., Pogo, A. O., Allfrey, V. G., Mirsky, A. E.: Changing patterns of histone acetylation and RNA synthesis in regeneration of the liver. Proc. nat. Acad. Sci. (Wash.) 59, 1337–1344 (1968)Google Scholar
- Ringertz, N. R.: Cytochemical properties of nuclear proteins and desoxyribonucleoprotein complexes in relation to nuclear function. In: Handbook of Molecular Cytology, A. Lima de Faria, p. 656–686. Amsterdam-London: North Holland Publ. Comp. 1969Google Scholar
- Steele, W. J., Busch, H.: Studies on acidic nuclear proteins of the Walker Tumor nuclei. Cancer Res. 23, 1153–1162 (1963)Google Scholar
- Zbarsky, I. B., Georgiev, S. P.: Cytological characteristics of protein and nucleoprotein fractions. Biochim. biophys. Acta (Amst.) 32, 301–305 (1959)Google Scholar