Biochemical Genetics

, Volume 23, Issue 1–2, pp 29–36 | Cite as

Electrophoretic and heat-stability polymorphism at the phosphoglucomutase (Pgm) locus in natural populations of Drosophila melanogaster

  • Ada Loverre-Chyurlia
  • George Carmody
Article

Abstract

Genetic polymorphism for electrophoretic and heat-sensitive alleles is known at the phosphoglucomutase (Pgm) locus in Drosophila melanogaster. Analysis of the distribution of electrophoretic and thermosensitive (ts) alleles was carried out in natural populations from Canada and West Africa and compared with already known data on Italian populations [Trippa, G., Loverre, A., and Catamo, A. (1976). Nature 260:42]. The data show the existence of five common alleles, Pgm1.00,tr, Pgm1,00,ts, Pgm0.70,ts, Pgm1.20,ts, and Pgm1.50,tr, and two rare alleles, Pgm0.55,ts and Pgm1.20,tr. The most frequent allele is always Pgm1.00,tr; the second most common allele is always of the ts type. The cumulated frequencies of ts alleles in the populations varies between 11 and 32%. The heat stability polymorphism is present in all populations examined and shows again the uniform geographic pattern that has been found for electrophoretic variation at this locus.

Key words

phosphoglucomutase Drosophila melanogaster thermostability allozymes geographic variation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderson, P. R., and Oakeshott, J. G. (1984). Parallel geographical patterns of allozyme variation in two sibling Drosophila species. Nature 308729.Google Scholar
  2. Ayala, F. J. (1983). Genetic polymorphism: From electrophoresis to DNA sequences. Experientia 39813.Google Scholar
  3. Bewley, G. C. (1978). Heat stability studies at the α-glycerophosphate dehydrogenase locus in populations of D. melanogaster. Biochem. Genet. 16769.Google Scholar
  4. Cavener, D. R., and Clegg, M. T. (1981). Temporal stability of allozyme frequencies in a natural population of Drosophila melanogaster. Genetics 98613.Google Scholar
  5. Cochrane, B. J. (1976). Heat stability variants of esterase-6 in Drosophila melanogaster. Nature 263131.Google Scholar
  6. Cochrane, B. J., and Richmond, R. C. (1979). Studies of esterase-6 in Drosophila melanogaster. II. The genetics and frequency distributions of naturally occurring variants studied by electrophoretic and heat stability criteria. Genetics 93461.Google Scholar
  7. David, J. R. (1982). Latitudinal variability of Drosophila melanogaster. Allozyme frequencies divergence between European and Afrotropical populations. Biochem. Genet. 20747.Google Scholar
  8. Franklin, I. R. (1981). An analysis of temporal variation at isozyme loci in D. melanogaster. In Gibson, J. B., and Oakeshott, J. G. (eds.), Genetic Studies of Drosophila Populations Australian National University, Canberra, pp. 217–236.Google Scholar
  9. Gosling, E. (1979). Hidden genetic variability in two populations of a marine mussel. Nature 279713.Google Scholar
  10. Oakeshott, J. G., Chambers, G. K., Gibson, J. B., and Willcocks, D. A. (1981). Latitudinal relationships of esterase-6 and phosphoglucomutase gene frequencies in Drosophila melanogaster. Heredity 47385.Google Scholar
  11. Poulik, M. D. (1957). Starch gel electrophoresis in a discontinuous system of buffer. Nature 1801477.Google Scholar
  12. Sampsell, B. (1977). Isolation and genetic characterization of alcohol dehydrogenase thermostability variants occurring in natural populations of Drosophila melanogaster. Biochem. Genet. 15971.Google Scholar
  13. Singh, R. S., Hickey, D. A., and David, J. (1982). Genetic differentiation between geographically distant populations of Drosophila melanogaster. Genetics 101235.Google Scholar
  14. Trippa, G., Loverre, A., and Catamo, A. (1976). Thermostability studies for investigating non-electrophoretic polymorphic alleles in Drosophila melanogaster. Nature 26042.Google Scholar
  15. Trippa, G., Catamo, A., Lombardozzi, A., and Cicchetti, R. (1978). A simple approach for discovering common non-electrophoretic enzyme variability: A heat denaturation study in Drosophila melanogaster. Biochem. Genet. 16299.Google Scholar
  16. Trippa, G., Scozzari, R., and Cicchetti, R. (1980). Dynamics of phosphoglucomutase heat sensitivity polymorphism in Culicidae. Experientia 36174.Google Scholar
  17. Trippa, G., Scozzari, R., Loverre, A., and Cicchetti, R. (1981). Temporal and spatial distribution of phosphoglucomutase (PGM) polymorphism in natural populations of Drosophila melanogaster. Experientia 37561.Google Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Ada Loverre-Chyurlia
    • 1
    • 2
  • George Carmody
    • 2
  1. 1.Dipartimento di Genetica e Biologia MolecolareCittà UniversitariaRomeItaly
  2. 2.Department of BiologyCarleton UniversityOttawaCanada

Personalised recommendations