Abstract
Backcross and F2 analyses have been carried out to determine the genetic basis of inheritance of three myosin light chain-1 variants present in the fast white muscle fibers of the domestic chicken. Two of the variants (types I and II) were described previously [Rushbrook, J. I., Yuan, A. I., and Stracher, A. (1982). Muscle Nerve 5:505], while the existence of the third (type III) is reported here. The results are consistent with an autosomal and allelic origin for the variants. A test linkage backcross to the muscular dystrophy gene am was found to be negative. This is the twelfth negative linkage result for the am gene.
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Asmunsden, V. S., and Julian, L. M. (1956). Inherited muscle abnormality in the domestic fowl. J. Hered. 47248.
Bugaisky, G., Alonso, S., Barton, P., Bugaisky, L., Cohen, A., Daubas, P., Guenet, J.-L., Minty, A., Robert, B., Weydert, A., and Buckingham, M. (1983). Structure and expression of actin and myosin genes in the mouse. J. Cell Biol. 97:329a.
Gauthier, G. F., and Lowey, S. (1977). Polymorphism of myosin among skeletal muscle types. J. Cell Biol. 74760.
Hudecki, M. S., and Barnard, E. A. (1976). Retardation of symptoms of dystrophy in genetically dystrophic chickens by chemotherapy. Res. Commun. Chem. Pathol. Pharmacol. 14167.
Matsuda, J., Maita, T., and Umegane, T. (1981). The primary structure of L-1 light chain of chicken fast skeletal muscle myosin and its genetic implication. FEBS Lett. 126111.
Nabeshima, Y., Fujii-Kuriyama, Y., Muramatsu, M., and Ogata, K. (1982). Molecular cloning and nucleotide sequences of the complementary DNAs to chicken skeletal muscle myosin two alkali light chain mRNAs. Nucleic Acids Res. 106099.
Nabeshima, Y., Fujii-Kuriyama, Y., Muramatsu, M., and Ogata, K. (1984). Alternative transcription and two modes of splicing result in two myosin light chains from one gene. Nature 308333.
Robert, B., Weydet, A., Caravatti, M., Minty, A., Cohen, A., Daubas, P., Gros, F., and Buckingham, M. (1982). cDNA recombinant plasmid complementary to mRNAs for light chains 1 and 3 of mouse skeletal muscle myosin. Proc. Natl. Acad. Sci. USA 792437.
Rushbrook, J. I., and Stracher, A. (1979). Comparison of adult, embryonic and dystrophic myosin heavy chains from chicken muscle by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and peptide mapping. Proc. Natl. Acad. Sci. USA 764331.
Rushbrook, J. I., Yuan, A. I., and Stracher, A. (1981). Abnormal myosin heavy chain variant associated with avian muscular dystrophy. Cell. Motil. 1399.
Rushbrook, J. I., Yuan, A. I., and Stracher, A. (1982) Two major allelic forms of myosin light chain-1 in strains of normal and dystrophic chickens. Muscle Nerve 5505.
Somes, R. G., Jr (1973). Linkage relationships in domestic fowl. J. Hered. 64217.
Wilson, B. W., Randall, W. R., Patterson, G. T., and Entrikin, R. K. (1979). Major physiological and histochemical characteristics of inherited dystrophy in the chicken. Ann. N.Y. Acad. Sci. 317224.
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This work was supported by grants from the Muscular Dystrophy Association, Inc., and the National Institutes of Health (R23 HL27883) to Dr. Rushbrook and from the National Institutes of Health (R01 NS18438) to Dr. A. Stracher. It is a joint publication from the institutions listed below and is Scientific Contribution No. 1,070 from the Storrs Agricultural Experiment Station.
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Rushbrook, J.I., Somes, R.G. Myosin light chain-1: Genetic analysis of three variants found in fast white chicken muscle and investigation of linkage with the muscular dystrophy gene. Biochem Genet 23, 17–27 (1985). https://doi.org/10.1007/BF00499109
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DOI: https://doi.org/10.1007/BF00499109