Advertisement

Biochemical Genetics

, Volume 24, Issue 5–6, pp 499–508 | Cite as

Distribution and probable physiological role of esterases in reproductive, digestive, and fat-body tissues of the adult cotton boll weevil, Anthonomus grandis Boh

  • Bobby R. Jones
  • Harold R. Bancroft
Article

Abstract

Polyacrylamide gel electrophoresis was used to examine gut, Malpighian tube, fat-body, testes, and ovariole tissues of the adult cotton boll weevil, Anthonomus grandis Boh. Esterases for which the inheritance has been reported previously by Terranova using whole-body homogenates were detected in dissected tissues and the probable physiological function of each allozyme is suggested. EST-1 occurs most frequently in ovarioles and female fat bodies. EST-2 is most often found in fat bodies and may be important in lipid turnover. No sex difference was observed. EST-3S is found in fat bodies and reproductive tissue, while EST-3F is always located in gut tissues, indicating that EST-3 is not controlled by a single autosomal locus with two codominant alleles as previously reported. EST-4, the most abundant esterase, can be detected in gut tissue at any age and is probably involved in digestion. EST-5 contains four allozymes which appear most frequently in testes and may be important during reproduction.

Key words

Anthonomus grandis cotton boll weevil esterase tissues inheritance allozymes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bancroft, H., and Jones, B. R. (1977). Genotypes of esterase II determined from frass of Anthonomus grandis Boh. (Coleoptera, Curculionidae). Biochem. Genet. 151175.Google Scholar
  2. Biggers, C. J., and Bancroft, H. R. (1977). Esterases of laboratory-reared and field-collected cotton boll weevils, Anthonomus grandis Boh.: Polymorphism of adult esterases and formal genetics of esterase II. Biochem. Genet. 15227.Google Scholar
  3. Brazzel, J. R., and Newson, L. D. (1959). Diapause in Anthonomus grandis Boh. J. Econom. Entomol. 52603.Google Scholar
  4. Cochrane, B. J., and Richmond, R. C. (1979). Studies of esterase 6 in Drosophila melanogaster. I. The genetics of a posttranslational modification. Biochem. Genet. 17167.Google Scholar
  5. E. C. Apparatus Corporation (1966). Buffer and Enzyme Incubation Recipes, Technical Bulletin 135.Google Scholar
  6. Finnerty, V., and Johnson, G. (1979). Post-translational modification as a potential explanation of high levels of enzyme polymorphism: Xanthine dehydrogenase and aldehyde oxidase in Drosophila melanogaster. Genetics 91695.Google Scholar
  7. Kambysellis, M. P., Johnson, F. M., and Richardson, R. H. (1968). Isozyme variability in species of the genus Drosophila. IV. Distribution of the esterases in the body tissues of D. aldrichi and D. Mulleri adults. Biochem. Genet. 1249.Google Scholar
  8. Peacock, A. C., Bunting, S. L., and Queen, K. C. (1965). Serum protein electrophoresis in acrylamide gel: Patterns from normal subjects. Science 1471451.Google Scholar
  9. Terranova, A. C. (1982). Inheritance of esterases in Anthonomus grandis grandis (Coleoptera, Curculionidae). Rev. Entomol. Soc. Am. 75261.Google Scholar
  10. Wagner, R. P., and Selander, R. K. (1974). Isozymes in insects and their significance. Annu. Rev. Entomol. 19117.Google Scholar

Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Bobby R. Jones
    • 1
  • Harold R. Bancroft
    • 2
  1. 1.Department of BiologyRhodes CollegeMemphis
  2. 2.Department of BiologyMemphis State UniversityMemphis

Personalised recommendations