Biochemical Genetics

, Volume 24, Issue 9–10, pp 643–656 | Cite as

Characterization of alcohol dehydrogenase in young soybean seedlings

  • Ryszard Brzezinski
  • Brian G. Talbot
  • Douglas Brown
  • Danuta Klimuszko
  • Stephen D. Blakeley
  • Jean-Paul Thirion
Article

Abstract

Molecular properties of alcohol dehydrogenase (ADH) were examined in young soybean seedlings. Soybean radicle tissue is ADH-rich. Enzyme specific activity decreases slowly with the development of roots and becomes almost undetectable when the first true leaves appear. Soybean ADH was not found to be inducible by flooding. 2,4-Dichlorophenoxyacetic acid (2,4-D) treatment increased ADH specific activity as much as 14-fold. Only one ADH isozyme was detected by isoelectric focusing. By DNA-DNA hybridization, soybean ADH genomic sequences were shown to be partly homologous to maize ADH1 cDNA. The presence of more than one Adh gene in soybean is discussed.

Key words

alcohol dehydrogenase soybean flooding isozymes DNA homology 

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Copyright information

© Plenum Publishing Corporation 1986

Authors and Affiliations

  • Ryszard Brzezinski
    • 1
  • Brian G. Talbot
    • 2
  • Douglas Brown
    • 3
  • Danuta Klimuszko
    • 1
  • Stephen D. Blakeley
    • 1
  • Jean-Paul Thirion
    • 1
  1. 1.Départment de Microbiologie, Faculté de MédicineUniversité de SherbrookeSherbrookeCanada
  2. 2.Départment de Biochimie, Faculté de MédicineUniversité de SherbrookeSherbrookeCanada
  3. 3.Department of BiologyBishop's UniversityLennoxvilleCanada

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