Abstract
A single genetic factor may affect the realization of several enzymes. To investigate the extent of pattern pleiotropy in the mouse, the activities of 28 enzymes in livers and brains from an inbred stock of C57BL/6J Nctr and five F1 stocks heterozygous for known electrophoretic variants were measured. Five congenic backcross stocks of C57BL/6J, each homozygous for one or more electrophoretic markers, were mated with C57BL/6J Nctr to construct the heterozygous variant F1 stocks. One of the five F1 stocks had no enzyme activities significantly different from those of C57BL/6J Nctr, while two had one enzyme, one had four enzymes, and another had six enzymes with activities that were significantly different from those of C57BL/6J Nctr. The latter two F1 stocks with multiple activity differences were those having the largest proportion of their genome of donor origin. Two of the F1 stocks were different from each other for one enzyme, and two were different for another enzyme. These differences and the relationship of these enzyme activities to the variant genes suggest that several genetic factors may affect an enzyme's realization.
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Bailey, D. W. (1977). Genetic drift: The problem and its possible solution by frozen-embryo storage. Ciba Found. Symp. 52291.
Bergmeyer, H. U. (1974). Nucleoside phosphorylase. In Methods of Enzymatic Analysis Academic Press, New York, Vol. 1, p. 490.
Dizik, M., and Elliot, R. W. (1978). A second gene affecting the sialylation of lysosomal α-mannosidase in mouse liver. Biochem. Genet. 16247.
Festing, M. (1973). A multivariate analysis of subline divergences in the shape of the mandible in C57BL/Gr mice. Genet. Res. Cambr. 21121.
Feuers, R. J., Delongchamp, R. R., Casciano, D. A., Burkhart, J. G., and Mohrenweiser, H. W. (1980). Assay for mouse tissue enzymes: Levels of activity and statistical variation for 29 enzymes of liver or brain. Anal. Biochem. 101123.
Hoi-Sen, Y. (1972). Is subline differentiation a continuing process in inbred strains of mice? Genet. Res. Cambr. 1953.
Kshirsagar, A. M. (1972). Regression and correlation among several variables. In Multivariate Analysis Mercel-Dekker, New York, pp. 9–17.
Lalley, P. A., and Shows, T. B. (1977). Lysosomal acid phosphatase deficiency: Liver specific variant in the mouse. Genetics 87305.
Paigen, K. (1971). The genetics of enzyme realization (a review). In Recheigl, M. (eds.), Enzyme Synthesis and Degradation in Mammalian Systems Karger, Basel, pp. 1–47.
Paigen, K. (1979). Acid hydrolases as models of genetic control. Annu. Rev. Genet. 13417.
Papaioannou, V. E., and Festing, M. F. W. (1980). Genetic drift in a stock of laboratory mice. Lab. Anim. 1411.
Roderick, T. R., and Womack, J. E. (1981). Strains of mice congenic for electrophoretic markers (in preparation).
Seal, H. L. (1968). Factor analysis. In Multivariate Statistical Analysis for Biologists Methuen, London, pp. 153–180.
Winer, B. J. (1971). Design and analysis of single-factor experiments. In Statistical Principles in Experimental Design McGraw-Hill, New York, pp. 149–201.
Womack, J. E., and Eicher, E. M. (1977). Liver specific lysosomal acid phosphatase deficiency (Apl) on mouse chromosome 17. Mol. Gen. Genet. 155315.
Womack, J. E., Davisson, M. T., Eicher, E. M., and Kendall, D. A. (1977). Mapping of nucleoside phosphorylase (Np-1) and esterase-10 on mouse chromosome 14. Biochem. Genet. 15347.
Womack, J. E., LuShun Yan, D., and Potier, M. (1981). Gene for neuraminidase activity on mouse chromosome 17 near H-2: Pleiotropic effect on multiple hydrolases. Science 212(3):63.
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This work was supported by the National Center for Toxicological Research and by Contract EV-76-S-02-3267 with the Department of Energy, Grant GM-19656 from the National Institute of General Medical Sciences, and Contract ES-42159 with the National Institute of Environmental Health Sciences.
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Feuers, R.J., Bishop, J.B., McGarrity, L.J. et al. Pleiotropic and other genetic effects influencing the activities of brain and liver enzymes in congenic lines of C57BL/6J mice with defined electrophoretic variant markers. Biochem Genet 20, 1139–1149 (1982). https://doi.org/10.1007/BF00498938
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DOI: https://doi.org/10.1007/BF00498938