Summary
Synaptic vesicles were isolated from the caudate nucleus of the pig by differential centrifugation and incubated with labelled monoamines in the absence or in the presence of ATP-Mg2+. Addition of ATP-Mg2+ enhanced the uptake of 14C-dopamine into the vesicles. Serotonin competitively inhibited the ATP-Mg2+-dependent uptake of 14C-dopamine without influencing the uptake which took place in the absence of ATP-Mg2+. Likewise, dopamine caused a dose-dependent inhibition of the ATP-Mg2+-dependent uptake of 14C-serotonin without inhibiting the uptake in the absence of ATP-Mg2+. Incubation of the vesicles with equal concentrations of 3H-dopamine and 14C-serotonin revealed that the presence of the one amine competitively inhibited the ATP-Mg2+-dependent uptake of the other. Tyramine competitively inhibited the ATP-Mg2+-dependent uptake of 14C-dopamine, 14C-serotonin and 14C-noradrenaline into the vesicles; the uptake of the amines which took place in the absence of ATP-Mg2+ was not impaired by tyramine. Analysis of the amine uptake by the ABC test showed that a mutual inhibition exists between dopamine and serotonin for the uptake into the synaptic vesicles. GABA did not influence the uptake of 14C-dopamine either in the absence, or in the presence of ATP-Mg2+.
The uptake of 14C-tyramine or 14C-β-phenylethylamine was not increased by ATP-Mg2+, while that of 3H-octopamine was greatly enhanced. Tyramine inhibited the ATP-Mg2+-dependent uptake of 3H-octopamine without influencing the uptake in the absence of ATP-Mg2+. During incubation with tyramine virtually no conversion to octopamine took place.
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This work was supported by the Deutsche Forschungsgemeinschaft and the Stiftung Volkswagenwerk.
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Matthaei, H., Lentzen, H. & Philippu, A. Competition of some biogenic amines for uptake into synaptic vesicles of the striatum. Naunyn-Schmiedeberg's Arch. Pharmacol. 293, 89–96 (1976). https://doi.org/10.1007/BF00498875
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DOI: https://doi.org/10.1007/BF00498875