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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 331, Issue 1, pp 23–26 | Cite as

Effect of short-term swimming stress and diazepam on 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) levels in the caudate nucleus: an in vivo voltammetric study

  • M. Ikeda
  • T. Nagatsu
Article

Summary

The effects of swimming stress on dopaminergic and serotonergic neurons were studied by an in vivo voltammetry technique. 3,4-Dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) levels in rat striatum were measured by differential pulse voltammetry with an electrochemically treated carbon fiber electrode. Exposure to swimming stress for 1 to 10 min to the animal increased the DOPAC and 5-HIAA peaks, which depended on the length of stress. Pretreatment of the rats with diazepam (10 mg/kg, i.p.) prevented completely the stress-induced increase in DOPAC levels but only partially reduced the increase in 5-HIAA levels.

Key words

In vivo voltammetry Swimming stress DOPAC 5-HIAA Diazepam 

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References

  1. Bliss EL, Ailion J, Zwanziger J (1968) Metabolism of norepinephrine and dopamine in rat brain with stress. J Pharmacol Exp Ther 164:122–134Google Scholar
  2. Cespuglio R, Faradji H, Riou F, Buda M, Gonon F, Pujol JF, Jouvet M (1981) Differential pulse voltammetry in brain tissue. II. Detection of 5-hydroxyindoleacetic acid and in the rat striatum. Brain Res 223:299–311Google Scholar
  3. Curzon G, Joseph MH, Knott PJ (1974) Effect of immobilization and food deprivation on rat brain tryptophan. J Neurochem 19:1967–1975Google Scholar
  4. Fadda F, Argiolas MR, Melis MR, Tissari AH, Onali PL, Gessa GL (1978) Stress-induced increase in 3,4-dihydroxyphenylacetic acid (DOPAC) levels in the cerebral cortex and in N. accumbens: reversal by diazepam. Life Sci 23:2219–2224Google Scholar
  5. Gonon F, Buda M, Cespuglio R, Jouvet M, Pujol JF (1980) In vivo electrochemical detection of catechols in the neostriatum of unanesthetized rats: dopamine or DOPAC? Nature 286:902–904Google Scholar
  6. Gonon F, Buda M, Cespuglio R, Jouvet M, Pujol JF (1981) Voltammetry in the striatum of chronic freely moving rats: Detection of catechols and ascorbic acid. Brain Res 223:69–80Google Scholar
  7. Gonon F, Buda M, Desimoni G, Pujol JF (1983) Catecholamine metabolism in the rat locus coeruleus as studied by in vivo differential pulse voltammetry. II. Pharmacological and behavioral study. Brain Res 273:207–216Google Scholar
  8. Ikeda M, Miyazaki H, Mugitani N, Matsushita A (1984a) Simultaneous monitoring of 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) levels in the brain of freely moving rats by differential pulse voltammetry technique. Neuroscience Res 1:171–184Google Scholar
  9. Ikeda M, Hirata Y, Fujita K, Shinzato M, Takahashi H, Yagyu S, Nagatsu T (1984b) Effects of stress on release of dopamine and serotonin in the striatum of spontaneously hypertensive rats: An in vivo voltammetry study. Neurochemistry International 6:509–512Google Scholar
  10. Joseph MH, Kennett AG (1983) Stress-induced release of 5-HT in the hippocampus and its dependence on increased tryptophan availability: An in vivo electrochemical study. Brain Res 270:251–257Google Scholar
  11. König JFR, Klippel RA (1963) The rat brain: A stereotaxic atlas of the forebrain and lower parts of the brain. Williams and Wilkins, Baltimore, MDGoogle Scholar
  12. Lavielle S, Tassin JP, Thiérry AM, Blanc G, Herve D, Barthelemy C, Glowinski J (1979) Blockade by benzodiazepines of the selective high increase in dopamine turnover induced by stress in mesocortical dopaminergic neurons of the rat. Brain Res 168:585–594Google Scholar
  13. Miyazaki H, Ikeda M, Mugitani N, Matsushita A (1983) In vivo voltammetric detection of DOPAC and 5-HIAA in the rat brain. Rev Polarography 29 Suppl.:108Google Scholar
  14. Nagatsu T, Ikeda M, Inagaki H, Rahman MH, Fujita K, Takahashi H, Shinzato M, Yagyu S (1984) Effect of stress on central monoaminergic neurons as measured by in vivo voltammetry and on serum aromatic L-amino acid decarboxylase activity of spontaneously hypertensive rats. In: Usdin E, Kvetnansky R, Axelrod J (eds). Gordon and Breach, New York, pp 425–433Google Scholar
  15. Reinhard JF, Bannon MJ, Roth RH (1982) Acceleration by stress of dopamine synthesis and metabolism in prefrontal cortex: antagonism by diazepam. Naunyn-Schmiedeberg's Arch Pharmacol 318:374–377Google Scholar
  16. Roth RH, Walters JR, Aghajanian GK (1973) Effect of impulse flow on the release and synthesis of dopamine in the rat striatum. In: Usdin E, Snyder SH (eds). Frontiers in catecholamine research. Pergamon Press, New York, pp 567–574Google Scholar
  17. Thiérry AM, Tassin JP, Blanc G, Glowinski J (1976) Selective activation of the mesocortical DA system by stress. Nature 263:242–243Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. Ikeda
    • 1
  • T. Nagatsu
    • 2
  1. 1.Division of Pharmacology, Shionogi Research LaboratoriesShionogi and Co.OsakaJapan
  2. 2.Laboratory of Cell Physiology, Department of Life Chemistry, Graduate School at NagatsutaTokyo Institute of TechnologyYokohama 227Japan

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