Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 331, Issue 1, pp 23–26 | Cite as

Effect of short-term swimming stress and diazepam on 3,4-dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) levels in the caudate nucleus: an in vivo voltammetric study

  • M. Ikeda
  • T. Nagatsu


The effects of swimming stress on dopaminergic and serotonergic neurons were studied by an in vivo voltammetry technique. 3,4-Dihydroxyphenylacetic acid (DOPAC) and 5-hydroxyindoleacetic acid (5-HIAA) levels in rat striatum were measured by differential pulse voltammetry with an electrochemically treated carbon fiber electrode. Exposure to swimming stress for 1 to 10 min to the animal increased the DOPAC and 5-HIAA peaks, which depended on the length of stress. Pretreatment of the rats with diazepam (10 mg/kg, i.p.) prevented completely the stress-induced increase in DOPAC levels but only partially reduced the increase in 5-HIAA levels.

Key words

In vivo voltammetry Swimming stress DOPAC 5-HIAA Diazepam 


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • M. Ikeda
    • 1
  • T. Nagatsu
    • 2
  1. 1.Division of Pharmacology, Shionogi Research LaboratoriesShionogi and Co.OsakaJapan
  2. 2.Laboratory of Cell Physiology, Department of Life Chemistry, Graduate School at NagatsutaTokyo Institute of TechnologyYokohama 227Japan

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