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Functional expression of P2Y purinoceptor in Xenopus oocyte injected with brain mRNA

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Abstract

Xenopus oocytes injected with embryonic guinea-pig brain mRNA expressed functional P2Y purinoceptors. Extracellular ATP stimulated in a dose-dependent manner a delayed Ca2+-dependent Cl− current component. Analysis of the interactions of ATP with compounds that affect Ca2+ fluxes through the plasma membrane or Ca2+ release from internal stores indicates that ATP raises [Ca2+]i by a mechanism that involves activation of voltage-dependent Ca2+ channels, which leads to influx of extracellular Ca2+ into the cells, as well as release of Ca2+ from intracellular stores. Since this phenomenon was not found in control oocytes, it is suggested that brain mRNA encoded for a newly synthesized Ca2+-release process stimulated by purinoceptor activation. This mechanism could be largely involved in the short-term regulation of intracellular Ca2+ level involved in ATP neuromodulation functions.

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Author notes

  1. E. Honoré

    Present address: Institut de Pharmacologie Moléculaire et Cellulaire, CNRS, 660 route des Lucioles, Sophia Antipolis, F-06560, Valbonne, France

Authors and Affiliations

  1. Laboratoire de Physiologie Cellulaire, UFR de Biologie, Université des Sciences et Techniques de Lille-Flandres-Artois, F-59655, Villeneuve d'Ascq Cédex, France

    E. Honoré, F. Fournier, T. Collin & P. Guilbault

  2. Centre de Recherches de Biochimie Macromoléculaire, CNRS, route de Mende, F-34033, Montpellier, France

    J. Nargeot

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  1. E. Honoré
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  2. F. Fournier
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  3. T. Collin
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  4. J. Nargeot
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  5. P. Guilbault
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Honoré, E., Fournier, F., Collin, T. et al. Functional expression of P2Y purinoceptor in Xenopus oocyte injected with brain mRNA. Pflugers Arch. 418, 447–452 (1991). https://doi.org/10.1007/BF00497772

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  • DOI: https://doi.org/10.1007/BF00497772

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