Skip to main content
SpringerLink
Log in

Regulation of potassium conductance by prostaglandins in cultured renal epitheloid (Madin-Darby canine kidney) cells

  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Abstract

Madin-Darby canine kidney (MDCK) cells form arachidonic acid metabolites following stimulation of several hormones known to modify the ion conductances at the plasma membrane. The present study has been performed to elucidate the influence of arachidonic acid on the electrical properties of subconfluent MDCK cells. As a result, arachidonic acid (1 or 10 μmol/l) leads to a transient hyperpolarization of the cell membrane, followed by a transient depolarization and a second, sustained hyperpolarization. The effects are inhibited by cycloxygenase inhibitor indomethacin (1 μmol/l). The initial transient hyperpolarization is mimicked by prostaglandin E2 (PGE2, 0.1 μmol/l), the sustained hyperpolarization by both PGE2 (0.1 μmol/l) and PGF2α (0.1 μmol/l). The transient hyperpolarization is paralleled by an increase of potassium selectivity and a decrease of cell membrane resistance and is thus the result of increased potassium conductance. The transient depolarization is paralleled by an increase of chloride selectivity, reflecting an increase of chloride conductance. The sustained hyperpolarization is paralleled by an increase of cell membrane resistance, and increase of potassium selectivity and a decrease of chloride selectivity, and is thus the result of decreasing chloride conductance. The observations reveal a role of prostaglandins in the regulation of ion conductances in MDCK cells, which could well participate in the transport regulation by hormones.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aboolian A, Vander Molen M, Nord EP (1989) Differential effects of phorbol esters on PGE2 and bradykinin-induced elevation of [Ca2+]i in MDCK cells. Am J Physiol 256:F1135-F1143

    Google Scholar 

  2. Besseghir K (1985) Renal tubular action of prostaglandin E2 on water and electrolyte excretion in the nonanaesthetized chicken. J Pharmacol Exp Ther 233:823–829

    Google Scholar 

  3. Bolivar JJ, Cereijido M (1987) Voltage and Ca2+-activated K+ channel in cultured epithelial cells (MDCK). J Membr Biol 97:43–51

    Google Scholar 

  4. Brown CDA, Simmons NL (1981) Catecholamine-stimulation of Cl-secretion in MDCK cell epithelium. Biochim Biophys Acta 649:427–435

    Google Scholar 

  5. Brown CDA, Simmons NL (1982) K+ transport in “tight” epithelial monolayers of MDCK cells: evidence for a calcium-activated K+ channel. Biochim Biophys Acta 690:95–105

    Google Scholar 

  6. Coyne DW, Mordhorst M, Bertrand W, Morrison AR (1989) Bradykinin stimulated PGE2 production is independent of changes in intracellular calcium in MDCK cells. Biochem Biophys Res Commun 161:1333–1340

    Google Scholar 

  7. Dominguez JH, Pitts TO, Brown T, Puschett DB, Schuler F, Chen TC, Puschett JB (1984) Prostaglandin E2 and parathyroid hormone: comparisons of their actions on the rabbit proximal tubule. Kidney Int 26:404–410

    Google Scholar 

  8. Dominguez JH, Schuler F, Olszowy MW, Brown T, Puschett JB (1988) Prostaglandin E2 is an inhibitor of adenylate cyclase in rabbit proximal tubule. Am J Physiol 254:C304-C309

    Google Scholar 

  9. Friedrich F, Paulmichl M, Kolb H-A, Lang F (1988) Inward rectifier K channels in renal epithelioid cells (MDCK) activated by serotonin. J Membr Biol 106:149–155

    Google Scholar 

  10. Friedrich F, Weiss H, Paulmichl M, Lang F (1989) Activation of potassium channels in renal epithelioid cells (MDCK) by extracellular ATP. Am J Physiol 256:C1016-C1021

    Google Scholar 

  11. Gstraunthaler G (1988) Epithelial cells in tissue culture. Renal Physiol Biochem 11:1–42

    Google Scholar 

  12. Hassid A (1983) Inhibition of prostaglandin biosynthesis in renal (MDCK) cells by cAMP. Am J Physiol 244:C369-C376

    Google Scholar 

  13. Hassid A (1983) Modulation of cyclic 3′5′-adenosine monophosphate in cultured renal (MDCK) cells by endogenous prostaglandins. J Cell Physiol 116:297–302

    Google Scholar 

  14. Iino Y, Brenner BM (1981) Inhibition of Na transport by prostaglandin (PGI2) in rabbit cortical collecting tubule. Prostaglandins 22:715–721

    Google Scholar 

  15. Jabs K, Zeidel ML, Silva P (1989) Prostaglandin E2 inhibits Na+-K+-ATPase activity in the inner medullary collecting duct. Am J Physiol 257:F424-F430

    Google Scholar 

  16. Kinoshita Y, Knox FG (1989) Role of prostaglandins in proximal tubule sodium reabsorption: response to elevated renal interstitial hydrostatic pressure. Circ Res 64:1013–1018

    Google Scholar 

  17. Kinoshita Y, Knox FG (1990) Mechanism of prostaglandin E2-induced increase of proximal sodium reabsorption in the rat. Am J Physiol 258:R82-R86

    Google Scholar 

  18. Kinoshita Y, Romero JC, Knox FG (1989) Effect of renal interstitial infusion of arachidonic acid on proximal sodium reabsorption. Am J Physiol 257:F237-F242

    Google Scholar 

  19. Kolb H-A, Paulmichl M, Lang F (1987) Epinephrine activates outward rectifying K channel in Madin-Darby canine kidney cells. Pflügers Arch 408:584–591

    Google Scholar 

  20. Kurtz A, Pfeilschifter J, Brown CDA, Bauer C (1986) NaCl transport stimulates prostaglandin release in cultured renal epithelial (MDCK) cells. Am J Physiol 250:C676-C681

    Google Scholar 

  21. Kurtz A, Pfeilschifter J, Malmström K, Woodson RD, Bauer C (1987) Mechanism of NaCl transport-stimulated prostaglandin formation in MDCK cells. Am J Physiol 252:C307-C314

    Google Scholar 

  22. Lang F, Friedrich F, Paulmichl M, Schobersberger W, Jungwirth A, Ritter M, Steidl M, Weiss H, Wöll E, Tschernko E, Paulmichl R, Hallbrucker C (1990) Ion channels in Madin-Darby canine kidney cells. Renal Physiol Biochem 13:82–93

    Google Scholar 

  23. Leighton JD, Pfeilschifter J (1990) Interleukin 1- and tumor necrosis factor-stimulation of prostaglandin E2 synthesis in MDCK cells, and potentiation of this effect by cycloheximide. FEBS Lett 259:289–292

    Google Scholar 

  24. Lewis MG, Spector AA (1981) Differences in types of prostaglandins produced by two MDCK canine kidney cell sublines. Prostaglandins 21:1025–1032

    Google Scholar 

  25. Lewis MG, Kaduce TL, Spector AA (1981) Effect of essential polyunsaturated fatty acid modifications on prostaglandin production by MDCK canine kidney cells. Prostaglandins 22:747–760

    Google Scholar 

  26. Madin SH, Darby NB (1958) As catalogued in: American Type Culture Collection Catalogue of Strains 2:574–576

    Google Scholar 

  27. Mené P, Dubyak GR, Scarpa A, Dunn MJ (1990) Regulation of cytosolic pH of cultured mesangial cells by prostaglandin F2α and thromboxane A2. Am J Physiol (in press)

  28. Miyanoshita A, Terada M, Endou H (1989) Furosemide directly stimulates prostaglandin E2 production in the thick ascending limb of Henle's loop. J Pharmacol Exp Ther 251:1155–1159

    Google Scholar 

  29. Nakao A, Allen ML, Sonnenburg WK, Smith WL (1989) Regulation of cAMP metabolism by PGE2 in cortical and medullary thick ascending limb of Henle's loop. Am J Physiol 256:C652-C657

    Google Scholar 

  30. Parker J, Daniel LW, Waite M (1987) Evidence of protein kinase C involvement in phorbol diester-stimulated arachidonic acid release and prostaglandin synthesis. J Biol Chem 262:5385–5393

    Google Scholar 

  31. Paulmichl M, Gstraunthaler G, Lang F (1985) Electrical properties of Madin-Darby canine kidney cells. Effects of extracellular potassium and bicarbonate. Pflügers Arch 405:102–107

    Google Scholar 

  32. Paulmichl M, Defregger M, Lang F (1986) Effects of epinephrine on electrical properties of Madin-Darby canine kidney cells. Pflügers Arch 406:367–371

    Google Scholar 

  33. Paulmichl M, Friedrich F, Lang F (1987) Effects of bradykinin on electrical properties of Madin-Darby canine kidney epithelioid cells. Pflügers Arch 408:408–413

    Google Scholar 

  34. Paulmichl M, Wöll E, Lang F (1988) Pertussis toxin-dependent and -independent hormonal effects on cultured renal epithelioid cells. FEBS Lett 234:263–266

    Google Scholar 

  35. Ritter M, Lang F (1990) Determination of cell membrane resistance in cultured renal epithelioid (MDCK) cells: effects of cadmium and mercury ions. Pflügers Arch 417:29–36

    Google Scholar 

  36. Roman RJ, Skelton M, Lechene C (1984) Prostaglandin-vasopressin interactions on the renal handling of calcium and magnesium. J Pharmacol Exp Ther 230:295–301

    Google Scholar 

  37. Schlondorff D, Satriano JA, Schwartz GJ (1985) Synthesis of prostaglandin E2 in different segments of isolated collecting tubules from adult and neonatal rabbits. Am J Physiol 248:F134-F144

    Google Scholar 

  38. Schwartzman M, Carroll MA, Ibraham NG, Ferreri NR, Songu-Mize E, McGiff JC (1985) Renal arachidonic acid metabolism. The third pathway. Hypertension 7:I136-I144

    Google Scholar 

  39. Simmons NL (1981) The action of prostaglandins upon a cultured epithelium of canine origin (MDCK). J Physiol (Lond) 310:28P-29P

    Google Scholar 

  40. Simmons NL, Brown CDA, Rugg EL (1984) The action of epinephrine on Madin-Darby canine kidney cells. Fed Proc 43:2225–2229

    Google Scholar 

  41. Slivka SR, Insel PA (1987) α 1-adrenergic receptor-mediated phosphoinositide hydrolysis and prostaglandin E2 formation in Madin-Darby canine kidney cells. Possible parallel activation of phospholipase C and phospholipase A2. J Biol Chem 262:4200–4207

    Google Scholar 

  42. Spry LA, Rapp NS, Thomasson DL, Zenser TV, Davis BB (1985) Effect of phosphodiesterase inhibitors on bradykinin-mediated prostaglandin E2 and cyclic AMP synthesis in renal papillary collecting tubule cells. Biochem Pharmacol 34:1565–1569

    Google Scholar 

  43. Torikai S, Kurokawa K (1983) Effect of PGE2 on vasopressin-dependent cell cAMP in isolated single nephron segments. Am J Physiol 245:F58-F66

    Google Scholar 

  44. Wald H, Scherzer P, Rubinger D, Popovtzer MM (1990) Effect of indomethacin in vivo and PGE2 in vitro on MTAL Na-K-ATPase of the rat kidney. Pflügers Arch 415:648–650

    Google Scholar 

  45. Weiss BA, Slivka SR, Insel PA (1989) Defining the role of protein kinase C in epinephrine- and bradykinin-stimulated arachidonic acid metabolism in Madin-Darby canine kidney cells. Mol Pharmacol 36:317–326

    Google Scholar 

  46. Wuthrich RP, Loup R, Favre L, Vallotton MB (1986) Dynamic response of PG synthesis to peptide hormones and osmolality in renal tubular cells. Am J Physiol 250:F790-F797

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute for Physiology, University of Innsbruck, Fritz-Pregl-Straße 3, A-6010, Innsbruck, Austria

    M. Steidl, M. Ritter & F. Lang

Authors
  1. M. Steidl
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Ritter
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Lang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Steidl, M., Ritter, M. & Lang, F. Regulation of potassium conductance by prostaglandins in cultured renal epitheloid (Madin-Darby canine kidney) cells. Pflugers Arch. 418, 431–436 (1991). https://doi.org/10.1007/BF00497769

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00497769

Key words

Search

Navigation