Summary
Cytoplasmic staining of glial cells by Alcian blue in 0.40 M magnesium chloride can be demonstrated in sections of brain tissue fixed in formol-acetic acid and in Bouin's solution. Phosphate-buffered aldehyde fixatives, whether at neutral or low pH, fail to preserve stainable material.
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Lai, M., Lampert, I.A. & Lewis, P.D. The influence of fixation on staining of glycosaminoglycans in glial cells. Histochemistry 41, 275–279 (1975). https://doi.org/10.1007/BF00497691
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DOI: https://doi.org/10.1007/BF00497691