Irradiation of cells in tissue culture
Cultures of human amnion were employed to check the hypothesis that cell strains with heteroploid chromosome counts regularly produce giant cells within 12 days following treatment with 2000 r and 4000 r of gamma irradiation from a cobalt source, while this response has not been obtained from primary cultures whose cells were presumed to be diploid.
The giant cell reaction not only was obtained from two transfer passage lines of a well-established amnion strain developed at Berkeley (No A 185-21C-26 and No A 185-21C-45) but was also found for a 20-day second passage culture of amnion. Since this line has continued to reproduce at a rapid rate, it is presumed to have assumed the features of a typical “strain” within the period of observation. This impression was reinforced by the finding that the chromosome number for 32 cells fixed on the 35th day had a modal value of 67.
In contrast, both untrypsinized and trypsinized spindle cells in primary cultures as well as unaltered epithelial elements which had not been subcultured gave no evidence of giant cell formation 12 days after exposure to 2000 r and 4000 r from a Cobalt60 source.
These data lend evidence that giant cell formation is related to the chromosomal constitution of the irradiated elements.
KeywordsCobalt Chromosome Number Giant Cell Primary Culture Gamma Irradiation
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- Berman, L., and C. S. Stulberg: Eight culture strains (Detroit) of human epitheliallike cells. Proc. Soc. exp. Biol. (N.Y.) 92, 730–735 (1956).Google Scholar
- Fernandes, M. V.: The development of a human amnion strain. Tex. Rep. Biol. Med. 16, 48–58 (1958).Google Scholar
- Fogh, J., and R. O. Lund: Continuous cultivation of epithelial cell strain (FL) from human amniotic membrane. Proc. Soc. exp. Biol. (N.Y.) 94, 532–537 (1957).Google Scholar
- Harper, J. Y., C. M. Pomerat and S. P. Kent: Irradiation of cells in tissue culture. IV. The effect of 2000 r and 4000 r of gamma irradiation from a Cobalt60 source on cultures of eye tissue. Z. Zellforsch. 47, 392–400 (1958).Google Scholar
- Hsu, T. C.: Mammalian chromosomes in vitro. I. The karyotype of man. J. Hered. 43, 167 to 172 (1952).Google Scholar
- Lahelle, O.: Preparation of monolayer tissue cultures from human amniotic membranes. Acta path. microbiol. scand. 39, 338–352 (1956).Google Scholar
- Makino, S., and T. Tanaka: Chromosome features in the regenerating rat liver following partial extirpation. Tex. Rep. Biol. Med. 11, 588–599 (1953).Google Scholar
- Paterson, E.: A comparison of the action of X and gamma radiation on fibroblasts. Brit. J. Radiol. 15, 257–263 (1942).Google Scholar
- Pomerat, C. M., S. P. Kent and L. C. Logie: Irradiation of cells in tissue culture. I. Giant cell induction in strain cultures versus elements from primary explants. Z. Zellforsch. 47, 158–174 (1957).Google Scholar
- Sachs, L., and M. C. Shelesnyak: The development and suppression of polyploidy in the developing and suppressed deciduoma in the rat. J. Endocr. 12, 146–151 (1955).Google Scholar
- Tanaka, T.: A study of the somatic chromosomes of rats. Cytologia (Tokyo) 18, 343–355 (1953).Google Scholar
- Therman, E.,and S. Timonen: Inconstancy of the human somatic chromosome complement. Hereditas (Lund) 37, 266–279 (1951).Google Scholar
- Timonen, S., and Eeva Therman: Variation of the somatic chromosome number in man. Nature (Lond.) 166, 995 (1950).Google Scholar
- Walker, B.: Polyploidy in transitional epithelium of the bladder in mice. Anat. Rec. 121, 379 (1955).Google Scholar
- Walker, B. E., and E. R. Boothroyd: Chromosome numbers in somatic tissues of mouse and man. Genetics 39, 210–219 (1954).Google Scholar
- Weinstein, H. J., C. Alexander, G. M. Yoshihara and W. M. M. Kirby: Preparation of amnion tissue culture. Proc. Soc. exp. Biol. (N.Y.) 92, 535–538 (1956).Google Scholar
- Westwood, J. C. N., I. A. MacPherson and D. H. J. Titmuss: Transformation of normal cells in tissue culture: Its significance relative to malignancy and virus vaccine production. Brit. J. exp. Path. 38, 138–154 (1957).Google Scholar
- Zitcer, E. M., J. Fogh and T. H. Dunnebacke: Human amnion cells for large scale production of polio virus. Science 122, 30 (1955).Google Scholar