Histochemistry

, Volume 74, Issue 4, pp 515–520 | Cite as

Pancreatic glucagon in human foetal stomach

  • A. M. J. Buchan
  • M. G. Bryant
  • B. A. Stein
  • M. Gregor
  • M. A. Ghatei
  • J. F. Morris
  • S. R. Bloom
  • J. M. Polak
Article

Summary

A combination of immunocytochemistry at light and electron microscopic levels, direct radioimmunoassay and measurement after gel chromatography have been used to identify and characterise a glucagon-like peptide detected in human foetal stomach. Immunocytochemistry, with region specific antisera, demonstrated that the glucagon-containing cells were indistinguishable from pancreatic A cells. Radioimmunoassay of tissue extracts confirmed the presence of significant quantities of glucagon, mean 21 pmol/g wet weight (range 14–29) in 16–26 week old foetuses, increasing to 41 pmol/g wet weight (range 31–52) in 33–30 week old foetuses and after gel chromatography the peptide was found to elute at the same position as standard porcine glucagon. It is apparent, therefore, that the human foetal fundus contains significant quantities of true pancreatic-type glucagon.

Keywords

Public Health Peptide Glucagon Microscopic Level Tissue Extract 

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • A. M. J. Buchan
    • 1
  • M. G. Bryant
  • B. A. Stein
    • 2
  • M. Gregor
    • 1
  • M. A. Ghatei
    • 1
  • J. F. Morris
    • 2
  • S. R. Bloom
    • 1
  • J. M. Polak
    • 1
  1. 1.Departments of Histochemistry and Medicine, Royal Postgraduate Medical SchoolHammersmith HospitalLondonEngland
  2. 2.Department of Human AnatomyOxfordEngland

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