Summary
Several technical difficulties have been overcome in the use of Lowicryl 4KM resin. In order to embed and section tissue satisfactorily in the resin, it has been found necessary to thoroughly degass the resin before infiltration and polymerisation. After irradiation with UV light, the blocks are further polymerised by exposure to daylight for 2–3 weeks and then stored under partial vacuum over dessicant.
This is a preview of subscription content, log in via an institution to check access.
References
Roth J, Bandayan M, Carlemalm E, Villiger W, Garavito M (1981) Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem 296:63–671
Bendayan M, Orstavik TB (1982) Immunocytochemical localization of kallikrein in the rat exocrine pancreas. J Histochem Cytochem 30:58–66
Bendayan M, Shore GE (1982) Immunocytochemical localisation of mitochondrial proteins in the rat hepatocyte. J Histochem Cytochem 30:139–147
Carlemalm E, Garavito RM, Villiger W (1982) Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc 126:123–143
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fryer, P.R., Wells, C. & Ratcliffe, A. Technical difficulties overcome in the use of Lowicryl 4KM EM embedding resin. Histochemistry 77, 141–143 (1983). https://doi.org/10.1007/BF00496645
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00496645