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Comparative evaluation of immunhistochemistry and enzyme histochemistry for granulocyte visualization in formalin-fixed and paraffin-embedded liver and lung biopsies

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Summary

Two methods for the specific visualization of granulocytes (PMNs) in formalin-fixed and paraffin-embedded tissue samples were compared: (1) the well-established enzyme histochemical method using naphthol-AS-D-chloracetate as a substrate; (2) an immunoperoxidase reaction using anti-PMN serum. Both methods are highly selective for visualizing PMNs. The advantages offered by the immunohistochemical method, i.e. better visualization of the remaining tissue structures and potential combination with other staining techniques, are stressed. Problems of PMN visualization in lung tissue and effects of tissue preparation are discussed. For histomorphometric studies the immunohistochemical method is preferable.

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Authors and Affiliations

  1. Pathology Unit, Landeskrankenhaus Oberwart, A-7400, Oberwart, Austria

    H. P. Dinges

  2. Ludwig Boltzmann Institute for Experimental Traumatology, Donaueschingenstrasse 13, A-1200, Vienna, Austria

    H. Redl

Authors
  1. H. P. Dinges
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  2. H. Redl
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Dinges, H.P., Redl, H. Comparative evaluation of immunhistochemistry and enzyme histochemistry for granulocyte visualization in formalin-fixed and paraffin-embedded liver and lung biopsies. Histochemistry 77, 9–14 (1983). https://doi.org/10.1007/BF00496631

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  • DOI: https://doi.org/10.1007/BF00496631

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