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Histochemistry

, Volume 89, Issue 1, pp 63–67 | Cite as

Scanning microfluorometric measurement of cell constituents

Principles of the method and its application to the determination of NAD content and redox state of XTH-2 cells in culture
  • J. Kajstura
  • J. Bereiter-Hahn
Article

Summary

The scanning of fluorescence of a cell culture along a path several milimeters long, gives a series of signals, which allow calculation of the fluorescence emitted per cell. The emission at 450±10 nm, excited at 360 nm, provides a measure of NADH (and NADPH) per cell. Combination of this method with modifications in energy metabolism, allows determination in the same sample of total NAD content, of NAD redox state, content of NAD in the mitochondria and content of NAD plus NADP in the cytoplasm. The method can be extended to measure other cellular constituents by labelling with fluorescent markers, e.g. antibodies.

Keywords

Public Health Cell Culture NADPH NADH Energy Metabolism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • J. Kajstura
    • 1
  • J. Bereiter-Hahn
    • 1
  1. 1.Cinematic Cell Research GroupJohann Wolfgang von Goethe-UniversitätFrankfurt a.M.Germany

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