Summary
Frozen sections of human, calf, rabbit, rat, cat, dog, goat, lamb, and hog corneas were stained with various lectins using an avidin-biotin-peroxidase complex to study glycoconjugates of stromal matrix. Staining of the stromal matrix and keratocytes with an α-galactose-specific lectin, Griffoma simplicifolia I (GSA-I) was species-dependent. The stromal matrices of cat, dog, and hog corneas invariably reacted intensely with this lectin, whereas those of the human, calf, rabbit, rat, and lamb did not react. A positive reaction with GSA-I could be abolished in each instance by preincubation of the sections with α-galactosidase. The stromal matrices and keratocytes of all nine species reacted positiyely with wheat germ agglutinin, concanavalin A, and Ricinus communis agglutinin but did not react with soybean agglutinin. Results of this study may help select and appropriate animal model to further investigate human corneal stromal glycoconjugates.
Similar content being viewed by others
References
Ahmed AI, Rahi AHS (1985) Physiological and pathobiological significance of ocular glycoproteins, studies using fluorescein labeled Glycine Max. Br J Ophthalmol 69:162–170
Bonvicini F, Versura P, Caruso F, Maltarello MC, Caramazza R, Laschi R (1983) Lectin receptors on human corneal epithelium. Visualization by fluorescence and electron microscopy. Cornea 2:237–241
Conen GR, Panjwani N, Balon B, Baum J (1985) Glycoconjugates of normal and wounded rabbit corneas. Invest Ophthalmol Vis Sci (Suppl) 26:177
Ecknardt AE, Goldstein IJ (1979) An α-d-galactopyranosyl containing glycoprotein from Ehrlich Ascites tumor cell plasma membranes. In: Gregory JD, Jeanloz RW (eds) Glycoconjugate research, vol 2. Academic Press, New York, pp 104–105
Eckhardt AE, Goldstein IJ (1983) Occurrence of α-d-galactosyl containing glycoproteins on Ehrlich tumor cell membranes Biochemistry 22:5280–5289
Gipson IK, Riddle CV, Kiorpes TC, Spurr SJ (1983) Lectin binding to cell surfaces: Comparisons between normal and migrating corneal epithelium. Dev Biol 96:337–345
Hayes CE, Goldstein IJ (1974) An α-d-galactopyfanosyl-binding-lectin from Bandeiraea simplicifolia seeds. J Biol Chem 249:1904–1914
Herold W (1972) Zum Nachweis der Blutgruppenantigene A und B in der menschlichen Hornhaut mittels der Immunofluoreszenz-Technik. Klin Monatsbl Angenheikd 161:658–662
Kress BC, Spiro RG (1984) Occurrence of α-d-galactosyl residues on the thyrotrophin receptor and other glycoconjugates of calf thyroid cell surface. Fed Proc 43:1706
Murphy LA, Goldstein IJ (1977) Five α-d-galactopyranosyl-binding isolectins from Bandeiraea simplicifolia seeds. J Biol Chem 252:4739–4742
Okada Y, Spiro RG (1980) Isolation and characterization of three major glycoproteins from thyroid plasma membranes. J Biol Chem 255:8865–8872
Panjwani N, Baum J (1985) Glycoproteins of bovine corncas. ARVO Abstracts. Invest Ophthalmol Vis Sci (Suppl) 26:178
Panjwani N, Rodrigues M, Free M, Baum J (1985) Localization of lectin binding sites in normal corneas and in corneas with lattice dystrophy. In: Davidson EA, Williams JC, DiFerrante NM (eds) Glycoconjugates Proceedings of the VIIIth International Symposium, vol 2, Sept 8–13, 1985 (Houston, TX) Pracger, New York, p 370
Panjwani N, Moulton P, Alroy J, Baum J (1986a) Localization of lectin binding sites in human, cat, and rabbit corneas. Invest Ophthalmol Vis Sci 27:1280–1284
Panjwani N, Rodrigues MM, Alroy J, Albert D, Baum J (1986b) Alteration in stromal glycoconjugates in macular corneal dystrophy. Invest Ophthalmol Vis Sci 27: 1211–1216
Peters BP, Goldstein IJ (1979) The use of fluorescein-conjugated Bandeiraea simplicifolia B4-isolectin as a histochemical reagent for the detection of α-d-galactopyranosyl groups. Exp Cell Res 120: 321–324
Shibata S, Peters BP, Roberts DD, Goldstein IJ, Liott LA (1982) Isolation of laminin by affinity chromatography on immobilized Griffonia simplicifolia lectin. FEBS Lett 142: 194–198
Spiro RG, Bhoyroo VD (1984) Occurrence of α-d-galactosyl residues in the thyroglobulins from several species. J Biol Chem 259: 9858–9866
Su-Ming H, Raine L (1982) Versatility of biotin-labeled lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections. J Histochem Cytochem 30: 157–161
Versura P, Bonvicini F, Vecci M, Caramazza R, Laschi R (1984/1985) Ultrastructural localization of Con A and WGA receptors on human corneal endothelium. Cornea 3: 192–196
Watkins WM (1966) Blood-group substances. Science 152: 172–181
Author information
Authors and Affiliations
Additional information
This investigation was supported by reasearch grants EY04034 and EY04819 from the National Institutes of Health, Bethesda MD
Rights and permissions
About this article
Cite this article
Panjwani, N., Baum, J. A histochemical comparison of human corneal stromal glycoconjugates with eight other species. Histochemistry 89, 41–45 (1988). https://doi.org/10.1007/BF00496582
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00496582