Summary
A sodium dependent GABA uptake system has been found in bovine adrenal medulla slices. This uptake has a K m of 83.19±38.45 μM and a V max of 9.20±1.36 pmol/min×mg of tissue. It was inhibited by nipecotic acid and 2,4-diaminobutyric acid (IC50 67 and 38.5 μM, respectively) but not by β-alanine at concentrations up to 5 mM, a result which is similar to those found for the neuronal GABA uptake rather than the glial uptake. It is suggested that GABA uptake together with catabolic action of GABA-transaminase, also found in this tissue, could be regulating the GABA levels disposable for the proposed modulator role on catecholamine secretion of this amino acid in adrenal medulla.
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Gasque, M.J., Cañadas Correas, S., Massó Córdoba, J.M. et al. Uptake of GABA by bovine adrenal medulla slices. Naunyn-Schmiedeberg's Arch. Pharmacol. 329, 372–375 (1985). https://doi.org/10.1007/BF00496370
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DOI: https://doi.org/10.1007/BF00496370